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Immediate early inflammatory gene responses of human umbilical vein endothelial cells to hemorrhagic venom

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Abstract

Objective and design

This report describes a focused immediate early gene response by human umbilical vein endothelial cells (HUVEC) to Echis carinatus snake venom.

Materials or subjects

Primary cultures of HUVEC were used to assess acute inflammatory gene responses.

Treatments

Crude E. carinatus venom (2.5 µg/ml) was used to stimulate HUVEC.

Results

HUVEC stimulated for 3 h with E. carinatus venom showed a focused response to the venom, with significant increases in metallothionein (e.g., MT1H, MT2A, MT1X) and cytochrome P450 (e.g., CYP1A1, CYP1B1) gene expressions compared to non-stimulated controls. Several other genes involved in cell growth and matrix attachment were repressed [e.g., thrombospondin 1 (THBS1), connective tissue growth factor (CTGF)].

Conclusions

These data suggest that acute vascular injury induced by hemorrhagic snake venom initiates an anti-oxidant response primarily involving metallothioneins.

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Authors and Affiliations

  1. Department of Biology and Physics, Kennesaw State University, 1000 Chastain Rd. Bldg 12 Rm 308, Kennesaw, GA, 30144, USA

    Eric A. Albrecht

  2. Michigan Center for Translational Pathology, University of Michigan Medical School, Ann Arbor, MI, 48109, USA

    Saravana M. Dhanasekaran & Scott Tomlins

Authors
  1. Eric A. Albrecht
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  2. Saravana M. Dhanasekaran
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  3. Scott Tomlins
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Correspondence to Eric A. Albrecht.

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Responsible editor: Makoto Katori.

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Albrecht, E.A., Dhanasekaran, S.M. & Tomlins, S. Immediate early inflammatory gene responses of human umbilical vein endothelial cells to hemorrhagic venom. Inflamm. Res. 60, 213–217 (2011). https://doi.org/10.1007/s00011-010-0286-1

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  • DOI: https://doi.org/10.1007/s00011-010-0286-1

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