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Cyanidin-3-O-β-glucoside inhibits LPS-induced expression of inflammatory mediators through decreasing IκBα phosphorylation in THP-1 cells

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Abstract

Objective and design

As a common phytochemical, cyanidin 3-O-β-glucoside (C3G) has a role in inhibiting inflammatory mediators; however, its mechanism of action remains unclear. The purpose of this study was to explore the effect of C3G on lipopolysaccharide (LPS)-stimulated TNFα and IL-6 expression in the human monocyte/macrophage cell line THP-1, and to explore the mechanisms involved.

Methods

Differentiated THP-1 cells were treated with different concentrations of C3G (0.005, 0.05, 0.5,10 μM) in the absence or presence of 1 ng/mL LPS. mRNA expression levels were detected by real time PCR, and secretion of TNFα and IL-6, phosphorylated IκBα, and nuclear factor-kappa B (NF-κB) P65 were monitored by ELISA or Western blotting analysis. The role of an inhibitor of IκBα phosphorylation, BAY 11-7082, in C3G inhibition of LPS-induced cytokines expression was investigated.

Results

C3G (0.05–0.5 μM) treatment significantly inhibited LPS-stimulated TNFα and IL-6 mRNA expression and secretion of these proteins by THP-1 cells. Phosphorylation of IκBα and NF-κB nuclear translocation could be blocked by 0.5 μM C3G. BAY 11-7082 treatment abolished C3G-induced reduction of TNFα and IL-6.

Conclusion

Our results suggest that C3G exerts its anti-inflammatory effect through inhibiting IκBα phosphorylation, thereby suppressing NF-κB activity in THP-1 cells.

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Acknowledgments

The authors thank Drs. Dayong Wu and Fu Shang in Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, USA for technical advice. This work was a joint research training program supported by the National Natural Science Foundation of China (Grant No. 30730079), and the U.S. Department of Agriculture (Grant No. 1950-51000-064).

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Authors and Affiliations

  1. Department of Nutrition, School of Public Health, Sun Yat-sen University (Northern Campus), 74 Zhongshan Road 2, Guangzhou, 510080, Guangdong, China

    Yinghui Zhang, Yanna Zhu, Min Xia, Qing Wang & Wenhua Ling

  2. Nutrition and Cancer Biology Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA, USA

    Fuzhi Lian & Xiang-Dong Wang

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  1. Yinghui Zhang
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Correspondence to Wenhua Ling or Xiang-Dong Wang.

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Responsible Editor: G. Wallace.

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Zhang, Y., Lian, F., Zhu, Y. et al. Cyanidin-3-O-β-glucoside inhibits LPS-induced expression of inflammatory mediators through decreasing IκBα phosphorylation in THP-1 cells. Inflamm. Res. 59, 723–730 (2010). https://doi.org/10.1007/s00011-010-0183-7

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  • DOI: https://doi.org/10.1007/s00011-010-0183-7

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