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Extramedullary hematopoiesis is dysregulated in histamine-free histidine decarboxylase knockout (HDC−/−) mice

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Abstract

Objective and design

In this study we investigated the role of histamine on the extramedullary hematopoiesis.

Methods

Male histidine decarboxylase knockout (HDC−/−) mice and wild-type mice were used (n = 5/group). Groups of mice received sublethal total-body gamma irradiation at a single dose of 4 Gy. Spleen cells were studied at different time points post-irradiation by flow cytometry, colony forming unit (CFU) assay, and real-time PCR. For statistical analysis Student’s t test, ANOVA, and Holm-Sidak post-hoc test were used.

Results

By day 14 after irradiation, spleen cell counts increased almost eightfold in wild-type and not even fourfold in HDC−/− mice (P < 0.01). The proliferative capacity and interleukin-3 signaling of stem cells were impaired in HDC−/− mice. STAT5 mRNA expression was decreased in granulocyte-myeloid colonies by 72.9 ± 8.6% (P < 0.001), compared to the wild-type.

Conclusions

The absence of histamine adversely affects splenic hematopoiesis via direct and indirect mechanisms.

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Acknowledgments

This work was supported by grant from the Hungarian National Scientific Research Foundation (OTKA73247).

Author information

Author notes

  1. Ivett Jelinek

    Present address: Experimental Immunology Branch, National Cancer Institute (NCI), US National Institutes of Health (NIH), 9000 Rockville Pike, Bethesda, MD, 20892, USA

Authors and Affiliations

  1. Department of Genetics, Cell- and Immunobiology, Semmelweis University, Nagyvárad tér 4, 1089, Budapest, Hungary

    Zsuzsanna Horváth, Ivett Jelinek, András Falus & Edit I. Buzás

  2. Research Group for Inflammation Biology and Immunogenomics, Hungarian Academy of Sciences, Semmelweis University, Nagyvárad tér 4, 1089, Budapest, Hungary

    Éva Pállinger & András Falus

  3. Department of Radiobiology, Research Institute of Military Medical Center, Hungarian Defence Forces, Róbert Károly krt. 44, 1134, Budapest, Hungary

    Győző Horváth

  4. Department of Radiation Medicine and Biodosimetry, ‘Frederic Joliot-Curie’ National Research Institute for Radiobiology and Radiohygiene, Anna u. 5, 1221, Budapest, Hungary

    Győző Horváth

  5. Clinical Laboratory of State Health Center, Róbert Károly krt. 44, 1134, Budapest, Hungary

    Gizella Veszely & József Fűrész

Authors
  1. Zsuzsanna Horváth
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  2. Éva Pállinger
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  3. Győző Horváth
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  4. Ivett Jelinek
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  5. Gizella Veszely
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  6. József Fűrész
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  7. András Falus
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  8. Edit I. Buzás
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Corresponding author

Correspondence to Edit I. Buzás.

Additional information

Responsible Editor: G. Wallace.

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Horváth, Z., Pállinger, É., Horváth, G. et al. Extramedullary hematopoiesis is dysregulated in histamine-free histidine decarboxylase knockout (HDC−/−) mice. Inflamm. Res. 59, 429–436 (2010). https://doi.org/10.1007/s00011-009-0114-7

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  • DOI: https://doi.org/10.1007/s00011-009-0114-7

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