Abstract
Objective and design
To investigate the modulating role of lymphocytes in leukocyte recruitment in a murine model of peritonitis.
Materials or subjects
RAG-1 knockout (KO) mice, NUDE mice and μMT KO mice were compared to their wild-type controls.
Treatment
Mice were administered with 1 ml of Brewer’s thioglycollate (BTG) and terminal peritoneal lavages were performed at 8, 24, 72 and 120 h after treatment.
Methods
Leukocyte numbers recruited at the different time points following a BTG administration were determined. Chemokine and cytokine levels were assessed by either ELISAs or cytometric bead array.
Results
RAG-1 KO mice (absent B and T cells) exhibited increased early neutrophil infiltration and blunted late monocyte/macrophage infiltration. NUDE mice (absent T cells) exhibited both increased neutrophil and monocyte/macrophage infiltration. In contrast, μMT KO mice (absent B cells) demonstrated reduced influx of both neutrophils and monocyte/macrophages. Chemokine analysis revealed various differences in important chemokines.
Conclusions
These data suggest that T cells act to suppress leukocyte recruitment while B cells promote leukocyte recruitment.
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Acknowledgments
J.-F. Cailhier is recipient of a scholarship and an operating grant from the Kidney Foundation of Canada. We thank Professor David Gray (Institute of Immunology and Infection Research, Edinburgh) for providing the μMT mice and Professor Sir John Savill (College of Medicine and Veterinary Medicine, Edinburgh) for thoughtful discussions. We also thank Spike Clay for expert technical assistance.
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Responsible Editor: G. R. Wallace.
J. Hughes and J.-F. Cailhier are joint senior authors.
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Kipari, T., Watson, S., Houlberg, K. et al. Lymphocytes modulate peritoneal leukocyte recruitment in peritonitis. Inflamm. Res. 58, 553–560 (2009). https://doi.org/10.1007/s00011-009-0019-5
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DOI: https://doi.org/10.1007/s00011-009-0019-5