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Increased leukotriene concentration× in submandibular glands from rats with experimental periodontitis

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Abstract

Objective and design

In the present study, we investigated the relation between the inflammatory mediators such as nitric oxide, prostaglandins, and cysteinyl-leukotrienes with mucin release and the sympathetic system in submandibular glands from rats with experimental periodontitis.

Materials or subjects

Submandibular glands from rats with experimental periodontitis.

Treatment

For the first experiment, rats were treated with hydrocortisone sc, 1 mg/kg for 3 days. All other experiments were carried out in isolated submandibular glands from untreated rats. Submandibular glands were treated with cysteinyl-leukotrienes, isoproterenol, NDGA, FPL 55712, L-NMMA, Nio, Nz, AMG, indomethacin, DuP 697 and atenolol.

Methods

Nitric oxide synthase activity, prostaglandin and cysteinyl-leukotriene productions and mucin secretion were determined. The Newman–Keuls statistical test was applied after analysis of variance.

Results

In rats with periodontitis hydrocortisone-induced a 36.6% (P < 0.05) decrease in mucin release. Only cysteinyl-leukotriene production was increased in rats with ligature (79.2%, P < 0.001). Either the inhibition of cysteinyl-leukotriene production or the block of leukotriene receptor abolished the increase in mucin secretion by 25.6% (P < 0.05) and 37% (P < 0.01), respectively, in glands from rats with ligature. On the other hand, the presence of cysteinyl-leukotrienes in the incubation medium induced mucin release from submandibular glands. Atenolol diminished by 24% (P < 0.05), the increase in cysteinyl-leukotrienes observed in rats with periodontitis. Besides, isoproterenol induced cysteinyl-leukotriene production in both groups.

Conclusion

In submandibular glands from rats with periodontitis, the increment in mucin release and cysteinyl-leukotrienes production are related events and both are associated with the sympathetic system.

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Acknowledgments

This work was supported by the Grants from University of Buenos Aires (UBACYT O 401), from the Argentinean Agency for the Promotion of Science and Technology (ANPCyT) and from the Argentinean National Research Council (CONICET). We thank Mrs. Elvita Vannucchi and Mrs. Elena Vernet for their outstanding technical assistance.

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Authors and Affiliations

  1. Pharmacology Unit, School of Dentistry, University of Buenos Aires, Buenos Aires, Argentina

    Lucila Busch, Valeria Miozza, Leonor Sterin-Borda & Enri Borda

  2. Argentine National Research Council (CONICET), Marcelo T de Alvear 2142 (1122AAH), Buenos Aires, Argentina

    Leonor Sterin-Borda & Enri Borda

  3. Cátedra de Farmacología. Facultad de Odontología, Universidad de Buenos Aires, Marcelo T. de Alvear 2142 (1122AAH), Buenos Aires, Argentina

    Lucila Busch

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  1. Lucila Busch
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  2. Valeria Miozza
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Correspondence to Lucila Busch.

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Responsible Editor: M. Parnham.

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Busch, L., Miozza, V., Sterin-Borda, L. et al. Increased leukotriene concentration× in submandibular glands from rats with experimental periodontitis. Inflamm. Res. 58, 423–430 (2009). https://doi.org/10.1007/s00011-009-0008-8

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  • DOI: https://doi.org/10.1007/s00011-009-0008-8

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