Abstract.
Neutral endopeptidase (NEP), which degrades substance P (SP), may regulate neutrophil activation during Mg-deficiency (MgD). Male Sprague-Dawley rats (180g) were fed MgD (~50 mg Mg/kg) or Mg-sufficient (MgS, 608 mg Mg/kg) diets for 7 days ± NEP inhibitor phosphoramidon (PR, 5 mg/kg/day, s.c.). MgD alone induced a 9-fold (vs. MgS, p <0.01) elevation in plasma SP; MgD+PR enhanced it further to 18-fold (p <0.001). Neutrophils from MgD+PR rats displayed a 3.9-fold higher (p <0.01) basal \({\rm O}_{2^-}\) generation, but those from MgD or PR alone were not activated. Plasma \({\rm PGE}_{2^-}\)metabolite levels rose 2.67- (p <0.01) and 1.56- (p <0.05) fold, respectively, in MgD+PR and MgD groups; the corresponding red blood cell glutathione levels were decreased 21% (p <0.025) and 7% (NS). MgD+PR significantly reduced neutrophil NEP activity by 48% (p <0.02); PR or MgD alone only reduced this activity 26% and 15%, respectively. We conclude that NEP inhibition potentiates SP-mediated neutrophil \({\rm O}_{2^-}\) production and may promote other inflammatory activities during MgD.
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Received 28 September 2007; returned for revision 22 November 2007; received from final revision 18 December 2007; accepted by G. Wallace 29 January 2008
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Mak, I.T., Kramer, J.H., Chmielinska, J.J. et al. Inhibition of neutral endopeptidase potentiates neutrophil activation during Mg-deficiency in the rat. Inflamm. res. 57, 300–305 (2008). https://doi.org/10.1007/s00011-007-7186-z
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DOI: https://doi.org/10.1007/s00011-007-7186-z