Abstract.
Objective and Design: Anti-CD45 antibody exhibits multiple biological effects on human mononuclear cells (MNC) and polymorphonuclear neutrophils (PMN). We intended to determine whether anti-CD45 antibody could affect arachidonic acid metabolism and thereby, the interactions between human naïve MNC and PMN.
Materials and Methods: Human naïve MNC and PMN were incubated with monoclonal anti-human CD45 IgG F(ab’)2 antibody or non-specific IgG F(ab’)2 for 30 min. The mRNA expression of cyclooxygenase type 1 (COX-1), type 2 (COX-2), 5-lipoxygenase (5-LOX) and leukotriene A4 hydrolase (LTA4 hydrolase) in both cells was detected by RT-PCR and quantified by densitometric determination. The presence of COX-1 and COX-2 molecules in the cells was detected by Western blot. The concentration of PGE2 and LTB4 in cultured supernatants was measured by EIA kits.
Results: Anti-CD45 IgG F(ab’)2 up-regulated LTA4 hydrolase mRNA expression and LTB4 production, but down-regulated COX-1 and COX-2 mRNA expression and PGE2 production, of naïve MNC compared to non-specific IgG F(ab’)2. In contrast, a reverse modulation by the specific antibody on PMN was observed including up-regulation of cyclooxygenase pathway and down-regulation of lipoxygenase pathway.
Conclusions: A novel activity of anti-CD45 with reverse modulation on cyclooxygenase/lipoxygenase pathways was found such that the expression of COX-1 and COX-2 in PMN, and 5-LOX and LTA4 hydrolase in MNC were enhanced.
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Received 4 May 2005; returned for revision 29 June 2005; returned for final revision 12 October 2005; accepted by M. Katori 16 November 2005
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Wu, C.H., Hsieh, S.C., Yu, H.S. et al. Anti-CD45 antibody enhances lipoxygenase pathway of human naïve mononuclear cells and cyclooxygenase pathway of neutrophils. Inflamm. res. 55, 92–98 (2006). https://doi.org/10.1007/s00011-005-0058-5
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DOI: https://doi.org/10.1007/s00011-005-0058-5