Skip to main content

Advertisement

SpringerLink
Log in

Diffusible signal to murine alveolar macrophages from lipopolysaccharide- and Escherichia coli-stimulated lung Type II epithelial cells

  • Original Paper
  • Published:
Inflammation Research Aims and scope Submit manuscript

Abstract.

Objective and design: To demonstrate a diffusible intercellular macrophage activation factor secreted by Type II alveolar epithelial cells (AECs) in transwell co-cultures.

Materials: T 7 , our Type II conditionally immortalized AEC line; MH-S, an alveolar macrophage cell line; Lipopolysaccharide (LPS) or uv-killed Escherichia coli (UVEC) for antigen presentation.

Methods: LPS or UVEC stimulation of T 7 cells in the lower chamber was investigated for ability to activate MH-S cells in the upper chamber, as assayed by nitric oxide production and western blots for inducible nitric oxide synthase-2.

Results: Both transwell and UVEC-conditioned medium experiments showed secretion of an MH-S activation factor by T 7 cells. Many common inflammatory cytokines were ruled out as this immunoactivator.

Conclusion: Demonstration of a diffusible activation factor produced by Type II AECs supports their potential role as first responders of innate immunity in the lung.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Department of Pathology, Saint Louis University Health Sciences Center, 3635 Vista Avenue, St. Louis, MO, 63110, USA

    M. M. Farberman, J. W. Hoffmann, J. S. Ryerse & D. E. deMello

  2. Department of Pediatrics, Saint Louis University Health Sciences Center, and the Pediatric Research Institute, Cardinal Glennon Children’s Hospital, St. Louis, MO, 63104, USA

    D. E. deMello

Authors
  1. M. M. Farberman
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. W. Hoffmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. S. Ryerse
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. D. E. deMello
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to D. E. deMello.

Additional information

Received 5 December 2003; returned for revision 9 January 2004; accepted by N. Boughton-Smith 21 April 2004

Rights and permissions

Reprints and permissions

About this article

Cite this article

Farberman, M.M., Hoffmann, J.W., Ryerse, J.S. et al. Diffusible signal to murine alveolar macrophages from lipopolysaccharide- and Escherichia coli-stimulated lung Type II epithelial cells. Inflamm. res. 53, 475–483 (2004). https://doi.org/10.1007/s00011-004-1289-6

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00011-004-1289-6

Search

Navigation