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The effect of nicotine on basophil histamine release

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Abstract

Background:Changes in the immune and inflammatory response are induced by smoking tobacco but underlying mechanisms remain to be elucidated.

Objective:This study investigated the effect of nicotine agonists on histamine release from human basophils.

Methods:Peripheral blood basophils were obtained from healthy volunteers. The effect of the nictotine agonists [–]-1-methyl-2-[3-pyridyl]pyrrolidine and (+)-nicotine di-p-toluoyltartrate salt on cell viability and anti-IgE induced histamine release was investigated.

Results:Cell viability was not altered by preincubation with the agents for 15 min. Anti-IgE induced histamine release was significantly inhibited by preincubation (15 min, 37°C) with [–]-1-methyl-2-[3-pyridyl]pyrrolidine at the highest concentration tested 10–3 M (p<0.01). Preincubation (15 min, 37°C) with (+)-nicotine di-p-toluoyltartrate salt significantly inhibited anti-IgE induced histamine release at 10–3M and 10–5 M (p<0.05).

Conclusions:This study has demonstrated that nicotine agonists inhibit histamine release from human basophils. Further studies examining the effect of smoking on basophil activation are required.

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Authors and Affiliations

  1. Departments of Clinical Biochemistry, Institute of Clinical Science, The Queen’s University of Belfast, Grosvenor Road, BT12 6BJ, Belfast, Northern Ireland, United Kingdom

    M. E. M. Thompson-Cree & M. Ennis

  2. Child Health, Institute of Clinical Science, The Queen’s University of Belfast, Grosvenor Road, BT12 6BJ, Belfast, Northern Ireland, United Kingdom

    M. D. Shields

  3. Department of Epidemiology & Public Health, Institute of Clinical Science, The Queen’s University of Belfast, Grosvenor Road, BT12 6BJ, Belfast, Northern Ireland, United Kingdom

    M. R. Stevenson

  4. School of Nursing & Midwifery, Medical Biology Centre, Queen’s University of Belfast, Lisburn Road, BT9 7BL, Belfast, Northern Ireland, United Kingdom

    M. E. M. Thompson-Cree

Authors
  1. M. E. M. Thompson-Cree
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  2. M. R. Stevenson
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  3. M. D. Shields
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  4. M. Ennis
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Corresponding author

Correspondence to M. E. M. Thompson-Cree.

Additional information

Received 28 September 2003; returned for revision 1 December 2003; accepted by A. Falus 23 December 2003

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Thompson-Cree, M.E.M., Stevenson, M.R., Shields, M.D. et al. The effect of nicotine on basophil histamine release. Inflamm. res. 53, 211–214 (2004). https://doi.org/10.1007/s00011-004-1249-1

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  • DOI: https://doi.org/10.1007/s00011-004-1249-1

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