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Profile of Inflammation-Associated Proteins in Early Post-Transplant Samples of Patients After Allogeneic Hematopoietic Stem Cell Transplantation: a Preliminary Study

  • Frantisek Mrazek
  • Petra Schneiderova
  • Eva Kriegova
  • Ludek Raida
  • Adam Kuba
  • Petr Gajdos
  • Nikola Königova
  • Jana Onderkova
  • Zuzana Ambruzova
Original Article
  • 259 Downloads

Abstract

Allogeneic hematopoietic stem cell transplantation (aHSCT) is used as a curative treatment in severe hematological and immunological disorders. Despite clear improvement of the aHSCT outcome, substantial proportion of patients still suffers from severe complications, including graft-versus-host disease (GvHD). The aim of this study was, therefore, to identify inflammation-associated molecules deregulated in the early serum samples of the patients after aHSCT and nominate markers associated with particular aHSCT parameters/complications. Serum concentrations of 92 inflammation-associated proteins were measured in samples obtained from 80 aHSCT patients 14 days after transplantation and from 23 healthy control subjects by a novel sensitive proximity extension assay technology using Proseek Multiplex Inflammation I kit. Serum profiles of inflammatory proteins in patients after aHSCT were substantially different from those observed in control subjects and related to underlying disease status before transplantation. Particularly, the difference between aHSCT patients and controls reached significance level for 57 analytes (40 upregulated, 17 downregulated in aHSCT patients). The concentration of several markers was associated with the level of donor/recipient HLA match (TGF-α: p corr = 0.025, HGF: p corr = 0.036) and with complete donor chimerism at day +30 after allografting (DNER: p corr = 0.042). None of the markers was significantly associated with acute and chronic GvHD after correction. More than half of investigated proteins significantly differed between the samples from aHSCT patients and healthy control subjects as a consequence of the “cytokine storm” after aHSCT. Comparisons of patient’s subgroups based on specific biological/clinical parameters revealed much less evident differences; nevertheless, we nominated several markers associated with the level of donor/recipient HLA match and post-transplant chimerism.

Keywords

Hematopoetic stem cell transplantation Graft-versus-host disease Protein profiles Biomarkers Inflammation 

Notes

Acknowledgements

Supported by the Ministry of Health of the Czech Republic projects IGA MH CZ NT12454-5 and MH CZ-DRO (FNOl, 00098892), and by Palacky University Olomouc project IGA_LF_2016_011.

Supplementary material

5_2016_446_MOESM1_ESM.doc (166 kb)
Supplementary material 1 (DOC 165 kb)

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Copyright information

© L. Hirszfeld Institute of Immunology and Experimental Therapy, Wroclaw, Poland 2016

Authors and Affiliations

  1. 1.Department of Immunology, Faculty of Medicine and DentistryPalacky University and University HospitalOlomoucCzech Republic
  2. 2.Department of Haemato-Oncology, Faculty of Medicine and DentistryPalacky University and University HospitalOlomoucCzech Republic
  3. 3.Department of Computer Science, Faculty of Electrical Engineering and Computer ScienceVSB-Technical University of OstravaOstravaCzech Republic

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