Abstract
Introduction
The aim was to evaluate the ability of egg yolk antibody (IgY) in blocking Staphylococcus aureus growth in vitro.
Materials and Methods
Specific IgY was produced by immunizing hens with formalin-killed S. aureus (ATCC 33593). Specific IgY against S. aureus was obtained from the yolks of their eggs with a carrageenan solution. IgY was identified by SDS-PAGE and Western blot and its activity against S. aureus was tested by ELISA. A growth inhibition assay and protein concentration determination were also conducted.
Results
ELISA indicated that the IgY was specific to the antigen; this activity was confirmed by Western blotting. The growth of S. aureus was inhibited by the specific IgY at concentrations of 1–5 μg/ml The bacteriostatic function of IgY appeared to result possibly from the interaction of IgY with surface components of S. aureus. In vitro experiments showed that the immunoglobulin from egg yolk interfered with the culture growth of the S. aureus.
Conclusion
These findings indicate that eggs from hens immunized with appropriate antigens are a potentially useful source of passive immunity.
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References
Akita EM, Nakai S (1992) Immunoglobulin from egg yolk: Isolation and purification. J Food Sci 57: 626–634
Allison JR (1985) Antibiotic residues in milk. Br Vet J 141: 9–16
Balaban N, Rasooly A (2000) Staphylococcal enterotoxins. Int J Food Microbiol 61: 1–10
Belschner AP, Hallberg JW, Nickerson SC et al (1996) Staphylococcus aureus mastitis therapy revisited. In Proc 35th Annu Mtg Natl Mastitis Council, p 116–122
Berghman LR, Abi-Ghanem D, Waghela SD et al (2005) Antibodies: an alternative for antibiotics?. Poult Sci 84: 660–666
Bohach GA, Foster TJ (2000) Staphylococcus aureus exotoxins. In: Fischetti VA, Novick RP, Ferretti JJ et al (eds) Gram-positive pathogens. ASM Press, Washington DC, pp 367–378
Booth JM, Harding F (1986) Testing for antibiotic residues in milk. Vet Rec 119: 565–569
Buchwald UK, Pirofski L (2003) Immune therapy for Infectious diseases at the dawn of the 21st century: the past, present and future role of antibody therapy, therapeutic vaccination and biological response modifiers. Curr Pharm Des 9: 945–968
Casadevall A (1996) Antibody-based therapies for emerging infectious diseases. Emerg Infect Dis 2: 200–208
Casadevall A, Scharff MD (1994) Serum therapy revisited: Animal models of infection and development of passive antibody therapy. Antimicrob Agents Chemother 38: 1695–1702
Chambers HF (2001) The changing epidemiology of Staphylococcus aureus?. Emerg Infect Dis 7: 178–182
Crossley KB, Archer GL (1997) The Staphylococci in Human Disease. Churchill Livingstone, New York, pp 565–582
Doyle ME (2001) Alternatives to Antibiotic Use for Growth Promotion in Animal Husbandry. FRI Briefings
Fioretto F, Cruz C, Largeteau A, Sarli TA et al (2005) Inactivation of Staphylococcus aureus and Salmonella enteritidis in tryptic soy broth and caviar samples by high pressure processing. Braz J Med Biol Res 38: 1259–1265
Fox LK, Bayles KW, Bohach GA (2000) Staphylococcal bovine mastitis. In: Honeyman A, Friedman H, Bendinelli M (eds) Staphylococcal infection and immunity. Kluwer Academic/Plenum Publishers, New York, pp 271–294
Hatta H, Kim M, Yamamoto T (1994) A novel isolation method for hen egg yolk antibody, “IgY”. Agric Biol Chem 54: 2531–2535
Jablonski LM, Bohach GA (2001) Staphylococcus aureus. In: Doyle MP, Beuchat LR, Montville TJ (eds) Food Microbiology: Fundamentals and Frontiers, 2nd edn. ASM Press, Washington DC, pp 410–434
Kirk JH, Berry IA, Gardner JM (2002) Dry cow antibiotic treatment in a herd with low contagious mastitis prevalence. In Proc 36th Annu Mtg Natl Mastitis Council, p 164
Lindberg E, Adlerberth I, Wold AE (2004) Antibiotic resistance in Staphylococcus aureus colonizing the intestines of Swedish infants. Clin Microbiol Infect 10: 890–894
Livemore D (2000) Antibiotic resistance in staphylococci. Int J Antimicrob Agents 16(suppl 1): S3–10
Marshall MJ, Bohach GA, Boehm DF (2000) Characterization of Staphylococcus aureus beta-toxin induced leukotoxicity. J Nat Toxins 9: 125–138
McEwen SA, Black WD, Meek AH (1992) Antibiotic residues (bacterial inhibitory substances) in the milk of cows under label and extra-label conditions. Can Vet J 33: 527–534
Mine Y, Kovacs-Nolan J (2002) Chicken egg yolk antibodies as therapeutics in enteric infectious disease: a review. J Med Food 5: 159–169
Moreillon P, Que YA, Glauser MP (2005) Staphylococcus aureus. In: Mandell GL, Bennett JE, Dolin R (eds) Principles and practice of infectious diseases, 6th edn. Elsevier Churchill Livingstone, Philadelphia, pp 2321–2351
Nickerson SC, Owens WE, Boddie RL (1995) Mastitis in dairy heifers: initial studies on prevalence and control. J Dairy Sci 78: 1607–1618
Oliver SP, Lewis MJ, Gillespie BE et al (1992) Influence of prepartum antibiotic therapy on intramammary infections in primigravid heifers during early lactation. J Dairy Sci 75: 406–414
Pereira MS, Leal NC, Leal TC et al (2002) Typing of human and bovine Staphylococcus aureus by RAPD-PCR and ribotyping-PCR. Lett Appl Microbiol 35: 32–36
Petrie A, Watson P (1999) Non-parametric statistical tests. In: Watson P (ed) Statistics for Veterinary and Animal Science. Blackwell Science, pp 138–152
Roberson JR, Fox LK, Hancock DD et al (1994) Ecology of Staphylococcus aureus isolated from various sites on dairy farms. J Dairy Sci 77: 3354–3364
Roghmann M, Taylor KL, Gupte A et al (2005) Epidemiology of capsular and surface polysaccharide in Staphylococcus aureus infections complicated by bacteraemia. J Hosp Infect 59: 27–32
Schade R, Calzado EG, Sarmiento R et al (2005) Chicken egg yolk antibodies (IgY-technology): a review of progress in production and use in research and human and veterinary medicine. Altern Lab Anim 33: 129–154
Schade R, Henklein P, Hlinak A (1997) Egg yolk antibody: state of the art and advantageous use in the life sciences. In: Animal Alternatives, Welfare and Ethics. Elsevier, Amsterdam, pp 973–981
Shin JH, Yang M, Nam SW et al (2002) Use of egg yolk-derived immunoglobulin as an alternative to antibiotic treatment for control of Helicobacter pylori infection. Clin Diagn Lab Immunol 9: 1061–1066
Sol J, Sampimon OC, Snoep JJ et al (1997) Factors associated with bacteriological cure during lactation after therapy for subclinical mastitis caused by Staphylococcus aureus. J Dairy Sci 80: 2803–2808
Sunwoo HH, Lee EN, Menninen K et al (2002) Growth inhibitory effect of chicken egg yolk antibody (IgY) on Escherichia coli O157:H7. J Food Sci 67: 1486–1494
Zhen YH, Jin LJ, Guo J et al (2008) Characterization of specific egg yolk immunoglobulin (IgY) against mastitis-causing Staphylococcus aureus. J Appl Microbiol 105: 1529–1535
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Guimarães, M.C.C., Amaral, L.G., Rangel, L.B.A. et al. Growth inhibition of Staphylococcus aureus by chicken egg yolk antibodies. Arch. Immunol. Ther. Exp. 57, 377–382 (2009). https://doi.org/10.1007/s00005-009-0041-x
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DOI: https://doi.org/10.1007/s00005-009-0041-x