Catabolic activity and biofilm formation of foodborne Listeria monocytogenes strains
- 9 Downloads
Listeria monocytogenes is a major foodborne pathogen causing increased morbidity worldwide. It forms resistant biofilm structures in food processing facilities after sanitization, consequently creating a public health concern. Many studies on the metabolism and transmission of L. monocytogenes has provided insights into its intracellular infection process, however there is limited understanding on the substrate utilization of the bacteria. Therefore, the main objective of this study was to investigate the carbon and nitrogen substrate catabolism and the biofilm forming potential of 3 Malaysian L. monocytogenes strains (LM41, LM92 and LM115) previously isolated from ready-to-eat foods. Biolog Phenotype Microarray (PM) system was used to study the catabolic activity of the foodborne strains in 190 carbon and 380 nitrogen sources. PM analysis showed that the carbon and nitrogen catabolic activity of L. monocytogenes strains were considerably limited and these strains utilised Tween 40 and Tween 80, which are commonly used for the sanitation in food and meat processing industries. Furthermore, all 3 strains showed strong biofilm forming potential in nutrient-rich and nutrient-limited media, irrespective of the serogroups. The data generated could be utilised to develop alternative measure to inhibit biofilm formation in L. monocytogenes in the food processing environment.
KeywordsBiofilm Foodborne pathogen Listeria monocytogenes Phenotype microarray Ready-to-eat food
We thank University of Malaya for the financial support and research facilities. This study was supported by the High Impact Research Grant UM.C/625/1/HIR/MOE/CHAN/01/02 from University of Malaya. The funders had no role in project design, data collection and analysis, decision to publish or preparation of the manuscript. This work was performed at the Laboratory of Biomedical Science and Molecular Microbiology, Institute of Graduate Studies, University of Malaya.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- CDC (2012) Multistate outbreak of listeriosis linked to whole cantaloupes from Jensen Farms, Colorado| Listeria| CDC. https://www.cdc.gov/listeria/outbreaks/cantaloupes-jensen-farms/index.html. Accessed 4 May 2018
- Giaouris EE, Simões MV (2018) Pathogenic biofilm formation in the food industry and alternative control strategies. In: Holban MA, Grumezescu MA (eds) Foodborne diseases: handbook of food bioengineering, 1st edn. Academic Press, Cambridge, Massachusetts, US, p 309–377. https://doi.org/10.1016/B978-0-12-811444-5.00011-7 CrossRefGoogle Scholar
- Jeyaletchumi P, Tunung R, Selina PM et al (2012) Assessment of Listeria monocytogenes in salad vegetables through kitchen simulation study. J Trop Agric Food Sci 40(1):55–62Google Scholar
- Wong WC, Pui CF, Chai LC et al (2011) Biosafety assessment of Listeria monocytogenes in vegetarian burger patties in Malaysia. Int Food Res J 18(1):459–463Google Scholar