SNP genotyping in sheep from northwest and east China for meat traceability
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Meat traceability is essential for meat safety and to solve adulteration issues. Single nucleotide polymorphisms (SNPs) are commonly used as a DNA marker and provide a way to trace meat and meat products. The high resolution melting method is a relatively cheap and fast method for detecting specific SNPs. In the present study, we selected 8 SNPs from the NCBI dbSNP database and tested their feasibility in blood or meat samples from 135 sheep reared in northwestern and eastern China. The results indicated that seven SNPs were suitable for meat traceability and the probability of 2 individuals having the same genotype was 1.85 out of 1000.
KeywordsDNA traceability SNPs HRM Sheep
This study was funded by the Grants 2012BAD28B02-03 and 2014BAD19B01 from the Ministry of Science and Technology, PR China.
Compliance with ethical standards
This study was funded by the Ministry of Science and Technology, PR China (Grant Number 2012BAD28B02-03 and 2014BAD19B01).
Conflict of interest
The authors declare that they have no conflict of interest.
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