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Incorporation and metabolism of trans 20∶5 in endothelial cells. Effect on prostacyclin synthesis

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Lipids

Abstract

To study the ability of long-chain trans fatty acids (FA) to be incorporated and metabolized into endothelial cells, bovine aortic endothelial cells were incubated with medium enriched eicosapentaenoic acid (EPA) bound to albumin (M2) or one of its geometrical isomers: 20∶5 5c,8c,11t,14c,17c(M3), 20∶5 5c,8c,11c,14c,17t(M4), or 20∶5 5c,8c,11t,14c,17t(M5). After 48 h of incubation, supernatant and cells were harvested and their lipids were analyzed, including prostacyclin synthesis. EPA and 22∶5n−3 of endothelial cells incubated with M2 were, respectively, three and two times higher than in control cells (incubated in M1, without any fatty acid added), whereas 22∶6n−3 increased only in the supernatant, suggesting its release after biosynthesis. However, 18∶2n−6 and 22∶4n−6 decreased (about 30%). Trans 20∶5 isomers represented 4.7, 3.9, and 5.2% of total phospholipid FA in endothelial cells incubated with M3, M4, and M5, respectively. They were elongated into trans 22∶5 and trans 24∶5, as revealed by gas chromatography-mass spectrometry and gas chromatography-Fourier transform infrared analysis. In cells incubated with M2, M3, M4, and M5, prostacyclin synthesis was inhibited by 49.0, 62.5, 60.5, and 72.0%, respectively. This effect may be due to less available arachidonic acid in the cells and to a competition between EPA isomers and AA at the level of cyclooxygenase pathway, as it was demonstrated that 20∶5 Δ17t was metabolized by this enzyme.

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Abbreviations

AA:

arachidonic acid

BAEC:

bovine aortic endothelial cells

DHA:

docosahexaenoic acid

DMOX:

dimethyloxazoline derivative

DPA:

docosapentaenoic acid

EPA:

eicosapentaenoic acid

FA:

fatty acids

FCS:

fetal calf serum

FTIR:

Fourier transform infrared

GC:

gas chromatography

HPLC:

high-performance liquid chromatography

NO:

nitric oxide

PG:

prostaglandin

PGI:

prostacyclin

RV:

retention volume

SFA:

saturated fatty acid

18∶3 Δ15t :

18∶3 9c,12c,15t

20∶5 Δ11t :

20∶5 5c,8c,11t,14c,17c

20∶5:

Δ17t, 20∶5 5c,8c,11c,14c,17t

20∶5 Δ11t :

20∶5 5c,8c,11t,14c,17t

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Author information

Authors and Affiliations

  1. Laboratoire de Recherche sur les Arômes, INRA, 21034, Dijon, France

    C. Ginies

  2. Service des Molécules Marquées, CEA-Saclay, 91191, Gifsur Yvette, France

    J. P. Noel

  3. Unité de Nutrition Lipidique, INRA, 17, rue Sully, BP 86510, 21065, Dijon Cedex, France

    C. Loï, J. M. Chardigny, C. Cordelet, L. Leclere, M. Genty & J. L. Sébédio

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  1. C. Loï
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  2. J. M. Chardigny
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  3. C. Cordelet
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  4. L. Leclere
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  5. M. Genty
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  8. J. L. Sébédio
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Correspondence to J. M. Chardigny.

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Loï, C., Chardigny, J.M., Cordelet, C. et al. Incorporation and metabolism of trans 20∶5 in endothelial cells. Effect on prostacyclin synthesis. Lipids 35, 911–918 (2000). https://doi.org/10.1007/S11745-000-0600-4

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  • DOI: https://doi.org/10.1007/S11745-000-0600-4

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