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Intravenously injected [1-14C]arachidonic acid targets phospholipids, and [1-14C]palmitic acid targets neutral lipids in hearts of awake rats

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Lipids

Abstract

The differential uptake and targeting of intravenously infused [1-14C]palmitic ([1-14C] 16∶0) and [1-14C]arachidonic ([1-14C]20∶4n−6) acids into heart lipid pools were determined in awake adult male rats. The fatty acid tracers were infused (170 μCi/kg) through the femoral vein at a constant rate of 0.4 mL/min over 5 min. At 10 min postinfusion, the rats were killed using pentobarbital. The hearts were rapidly removed, washed free of exogenous blood, and frozen in dry ice. Arterial blood was withdrawn over the course of the experiment to determine plasma radiotracer levels. Lipids were extracted from heart tissue using a two-phase system, and total radioactivity was measured in the nonvolatile aqueous and organic fractions. Both fatty acid tracers had similar plasma curves, but were differentially distributed into heart lipid compartments. The extent of [1-14C]20∶4n−6 esterification into heart phospholipids, primarily choline glycerophospholipids, was elevated 3.5-fold compared to [1-14C]16∶0. The unilateral incorporation coefficient, k *, which represents tissue radioactivity divided by the integrated plasma radioactivity for heart phospholipid, was sevenfold greater for [1-14C]20∶4n−6 than for [1-14C]16∶0. In contrast, [1-14C]16∶0 was esterified mainly into heart neutral lipids, primarily triacylglycerols (TG), and was also found in the nonvolatile aqueous compartment. Thus, in rat heart, [1-14C]20∶4n−6 was primarily targeted for esterification into phospholipids, while [1-14C]16∶0 was targeted for esterification into TG or metabolized into nonvolatile aqueous components.

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Abbreviations

CE:

cholesteryl esters

CerPCho:

sphingomyelin

ChoGpl:

choline glycerophospholipids

DG:

diacylglycerols

EtnGpl:

ethanolamine glycerophospholipids

FFA:

free fatty acids

IL-1β:

interleukin-1β

k * :

unilateral incorporation coefficient

PET:

positron emission tomography

PlsCho:

choline

PlsEtn:

ethanolamine plasmalogen

PtdIns:

phosphatidylinositol

PtdOH:

phosphatidic acid

PtdSer:

phosphatidylserine

TG:

triacylglycerols

TLC:

thin-layer chromatography

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Authors and Affiliations

  1. Section on Brain Physiology and Metabolism, National Institute on Aging, National Institutes of Health, Building 10, Room 6C103, 20892, Bethesda, MD

    Eric J. Murphy (National Research Council Senior Fellow), Thad A. Rosenberger, Casey B. Patrick & Stanley I. Rapoport

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  1. Eric J. Murphy
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  2. Thad A. Rosenberger
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  3. Casey B. Patrick
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  4. Stanley I. Rapoport
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Correspondence to Eric J. Murphy.

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Murphy, E.J., Rosenberger, T.A., Patrick, C.B. et al. Intravenously injected [1-14C]arachidonic acid targets phospholipids, and [1-14C]palmitic acid targets neutral lipids in hearts of awake rats. Lipids 35, 891–898 (2000). https://doi.org/10.1007/S11745-000-0598-7

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  • DOI: https://doi.org/10.1007/S11745-000-0598-7

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