Abstract
Earlier, we detected antiinflammatory action of comaruman, pectin of the marsh cinquefoil Comarum palustre L. This effect can be explained by new data concerning inhibition of adhesion of human neutrophils to fibronectin by comaruman and its fragments. The galacturonan backbone fragment of molecular mass >10 kD appears to be the active region of the comaruman macromolecule. Comaruman CP (50–200 μg/ml) was found to decrease adhesion of neutrophils stimulated by phorbol 12-myristate 13-acetate (PMA, 1.625 μM) and by dithiothreitol (DTT, 0.5 mM). The fragments of comaruman CP-H (100 kD), CP-H1 (10-50 kD), and CP-H2 (100 kD) obtained by acidic hydrolysis and representing regions of linear polygalacturonan are shown to inhibit neutrophil adhesion more than the crude pectin. A fragment CP-E (<10 kD) obtained using pectinolysis and representing a branched region of the comaruman macromolecule failed to influence cell adhesion. The parent comaruman CP as well as fragments of its polygalacturonan backbone diminish PMA-initiated generation of oxygen radicals in neutrophils.
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Translated from Biokhimiya, Vol. 70, No. 1, 2005, pp. 133–138. Original Russian Text Copyright © 2005 by Popov, Ovodova, Popova, Nikitina, Ovodov. Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM04-202, October 24, 2004.
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Popov, S.V., Ovodova, R.G., Popova, G.Y. et al. Adhesion of human neutrophils to fibronectin is inhibited by comaruman, pectin of marsh cinquefoil comarum palustre L., and by its fragments. Biochemistry (Moscow) 70, 108–112 (2005). https://doi.org/10.1007/PL00021770
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DOI: https://doi.org/10.1007/PL00021770