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Spatial Coordination of Chloroplast and Plasma Membrane Activities in Chara Cells and Its Disruption through Inactivation of 14-3-3 Proteins

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Abstract

In Chara corallina cells exposed to continuous light, external pH (pHo) and photosystem II (PSII) photochemical yield show correlated banding patterns. Photosynthetic activity is low in cell regions producing alkaline zones and high in the acid regions. We addressed the question whether (and how) photosynthetic activity and plasma membrane (PM) H+-pumping and H+-conductance are coupled in the different bands. First, PM H+-pump activity was stimulated with fusicoccin. This resulted in a more acidic pH in the acid bands without disturbing the correlation of photosynthetic electron transport and H+ fluxes across the PM. Next, H+-pump activity was reduced through microinjection of a phosphorylated peptide matching the canonical 14-3-3 binding motif RSTpSTP in the acid cell region. Microinjection induced a rapid (~5 min) rise in pHo by ca. 1.0 unit near the injection site, whereas the injection of the non-phosphorylated peptide had no effect. This pH rise confirms the supposed inhibition of the H+-pump upon the detachment of 14-3-3 proteins from the H+-ATPase. However, the PSII yield in the cell regions corresponding to the new alkaline peak remained high, which violated the normal inverse relations between the pHo and PSII photochemical yield. We conclude that the injection of the competitive inhibitor of the H+ATPase disrupts the balanced operation of PM H+-transport and photosynthetic electron flow and promotes electron flow through alternative pathways.

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Abbreviations

NR:

nitrate reductase

NR-C:

non-phosphorylated peptide matching the 14-3-3 interaction domain in barley nitrate reductase

NR-P:

a similar peptide containing phosphorylated serine

pHo :

external pH near the cell surface

PM:

plasma membrane

PSII:

photosystem II

ΔF/F ’m :

effective quantum yield of PS II photochemistry

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Authors and Affiliations

  1. Department of Biophysics, Faculty of Biology, Lomonosov Moscow State University, 119992, Moscow, Russia

    A. A. Bulychev

  2. Department of Developmental Genetics, Vrije Universiteit, Faculty of Earth and Life Sciences, De Boelelaan 1085, 1081, HV Amsterdam, The Netherlands

    P. W. J. van den Wijngaard & A. H. de Boer

Authors
  1. A. A. Bulychev
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  2. P. W. J. van den Wijngaard
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  3. A. H. de Boer
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Correspondence to A. A. Bulychev.

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Translated from Biokhimiya, Vol. 70, No. 1, 2005, pp. 68–76. Original Russian Text Copyright © 2005 by Bulychev, van den Wijngaard, de Boer. Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM04-068, October 31, 2004.

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Bulychev, A.A., van den Wijngaard, P.W.J. & de Boer, A.H. Spatial Coordination of Chloroplast and Plasma Membrane Activities in Chara Cells and Its Disruption through Inactivation of 14-3-3 Proteins. Biochemistry (Moscow) 70, 55–61 (2005). https://doi.org/10.1007/PL00021756

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  • DOI: https://doi.org/10.1007/PL00021756

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