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Model description of bacterial 3-methylcatechol production in one- and two-phase systems

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Abstract

Pseudomonas putida MC2 produces 3-methylcatechol from toluene in aqueous medium. A second phase of 1-octanol may improve total product accumulation. To optimise the design of such a biphasic process, a process model was developed, both for one- and two-phase applications. The insights obtained by the model predictions showed the importance of different process parameters (like growth substrate concentration and partition coefficient) on growth of biomass, accumulation of 3-methylcatechol and processing time. For future applications, the process model can be used to ensure enough extraction capacity from aqueous to octanol phase. It is a useful tool to define the optimum process conditions, depending on the desired optimisation parameter: product concentration or processing time.

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Acknowledgements

This work was carried out as part of the Innovation Oriented research Program on Catalysis (IOP Katalyse, No. IKA96003) sponsored by the Netherlands Ministry of Economic Affairs.

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Author notes

  1. J. A. M. de Bont

    Present address: TNO MEP, P.O. Box 342, 7300 AH, Apeldoorn, The Netherlands

Authors and Affiliations

  1. Food and Bioprocess Engineering Group, Department of Agrotechnology and Food Sciences, Wageningen University, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands

    L. E. Hüsken, J. Hoogakker, J. A. M. de Bont, J. Tramper & H. H. Beeftink

Authors
  1. L. E. Hüsken
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  2. J. Hoogakker
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  3. J. A. M. de Bont
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  4. J. Tramper
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  5. H. H. Beeftink
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Corresponding author

Correspondence to H. H. Beeftink.

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Hüsken, L.E., Hoogakker, J., de Bont, J.A.M. et al. Model description of bacterial 3-methylcatechol production in one- and two-phase systems. Bioprocess Biosyst Eng 26, 11–17 (2003). https://doi.org/10.1007/PL00021284

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  • DOI: https://doi.org/10.1007/PL00021284

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