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Chromosomal effects on peptidase activities inDrosophila melanogaster

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Abstract

The peptidase system inDrosophila melanogaster (dipeptidase-A, -B, and -C and leucine aminopeptidases G and P) was used as a model to study the effects of modifier genes on activity of enzymes with similar functions. A screen of X, second, and third chromosome substitution isogenic lines revealed the presence of activity modifiers for peptidases on all three chromosomes. Correlation analyses indicated that covariation between some of the peptidase activities is independent of genetic background, while others are associated with variable second chromosomes. Chromosome-specific effects onK m ,V max, and specific activity of partially purified peptidases were also detected. Moreover, a repeatable technique using anion-exchange column chromatography allowed the characterization of possibly two putative peptidic enzymes, glycyl-l-isoleucine-ase andl-leucyl-l-proline-ase, whose kinetic properties differ from the dipeptidases and the leucine aminopeptidases. These findings confirm the existence of activity modifiers for peptidases, much like other enzymes inDrosophila melanogaster.

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Author notes

  1. Karen D. Mathes

    Present address: ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, 21702, Frederick, Maryland

  2. Christo I. Shalish

    Present address: Molecular Neurogenetics Unit (Neuroscience Center), Massachusetts General Hospital East, 02129, Charleston, Massachusetts

Authors and Affiliations

  1. Department of Biology, Gettysburg College, 17325, Gettysburg, Pennsylvania

    Kazuo Hiraizumi

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  1. Kazuo Hiraizumi
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  2. Karen D. Mathes
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  3. Christo I. Shalish
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Additional information

These studies were supported by grants from the National Institutes of Health (GM42-115-01A1), the Whitaker Foundation of the Research Corporation (C-2560), and the National Science Foundation (USE 8951018) to Kazuo Hiraizumi.

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Hiraizumi, K., Mathes, K.D. & Shalish, C.I. Chromosomal effects on peptidase activities inDrosophila melanogaster . Biochem Genet 31, 29–50 (1993). https://doi.org/10.1007/PL00020384

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  • DOI: https://doi.org/10.1007/PL00020384

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