Abstract
Fish arylsulfatases (arylsulfate sulfohydrolase; EC 3.1.6.1) were resolved into cationic arylsulfatase A-like (ARSA) and anionic arylsulfatase B-like (ARSB) fractions by DEAE-Sephacel chromatography. Green sunfish (GSF) hepatic ARSA was more acidic and more thermostable than bluegill (BG) ARSA. GSF × BG interspecific hybrids preferentially expressed GSF ARSA, while BG × GSF hybrids appeared to produce a dimeric enzyme consisting of both GSF and BG ARSA polypeptides. GSF hepatic betaglucuronidase (GUS) also proved to be more thermostable than BG GUS. Thermostabilities of GUS produced by reciprocal interspecific hybrids were very similar to that of GSF GUS. Either GSF GUS is preferentially expressed in both interspecific hybrids or both the GSF and BG GUS polypeptides are synthesized in comparable amounts, and the GSF GUS polypeptide sufficiently stabilizes the heterotetramers produced by the hybrids to produce denaturation profiles closely approximating that of the GSF enzyme.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Beltramini-Guarini, P., Gitzelmann, R., and Pfister, K. (1984). Presence and absence of the microsomal beta-glucuronidase in mice correlates with differences in the processing of the lysosomal enzyme.Eur. J. Cell Biol. 34165.
Bracey, L. T., and Paigen, K. (1987). Changes in translational yield regulate tissue-specific expression of beta-glucuronidase.Proc. Natl. Acad. Sci. USA 849020.
Cini, J. K., Cook, P. F., and Gracy, R. W. (1988). Molecular basis for the isozymes of bovine glucose-6-phosphate isomerase.Arch. Biochem. Biophys. 29396.
Daniel, W. L., Ruoff, B. M., Thompson, D. B., and Gore, J. B. (1985). Purification and properties of arylsulfatase B from high- and low-activity mouse strains.Biochem. Genet. 23771.
Daniel, W. L., Leonard, L., and Carmody, T. (1987). Comparative biochemistry of fish and amphibian arylsulfatases.Comp. Biochem. Physiol. 87B385.
Little, L. E., Lau, M. M. H., Quon, D. V. K., Fowler, A. V., and Neufeld, E. F. (1988). Proteolytic processing of the alpha-chain of the lysosomal enzyme, beta-hexosaminidase, in normal human fibroblasts.J. Biol. Chem. 2634288.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. L. (1951). Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193267.
Mahuran, D. J., Neote, K., Klavins, M. H., Leung, A., and Gravel, R. A. (1988). Proteolytic processing of pro-alpha and pro-beta precursors from human beta-hexosaminidase.J. Biol. Chem. 2634612.
Paigen, K. (1979). Acid hydrolases as models of genetic control.Annu. Rev. Genet. 13417.
Palmer, R., Gallagher, P. M., Boyko, W. L., and Ganschow, R. E. (1983). Genetic control of levels of murine kidney glucuronidase mRNA in response to androgen.Proc. Natl. Acad. Sci. USA 807596.
Parker, H. R., Philipp, D. P., and Whitt, G. S. (1985). Relative developmental success of interspecificLepomis hybrids as an estimate of gene regulatory divergence between species.J. Exp. Zool. 233451.
Pasdar, M., Philipp, D. P., Mohammad, W. A., and Whitt, G. S. (1984). Differences in tissue expressions of enzyme activities in interspecific sunfish (Centrarchidae) hybrids and their backcross progeny.Biochem. Genet. 22931.
Pasdar, M., Philipp, D. P., and Whitt, G. S. (1984). Linkage relationships of nine enzyme loci in sunfishes (Lepomis; Centrarchidae).Genetics 107435.
Philipp, D. P., Parker, H. R., and Whitt, G. S. (1983). Evolution of gene regulation: Isozymic analysis of patterns of gene expression during hybrid fish development. In Rattazzi, M. C., Scandalios, J. G., and Whitt, G. S. (eds.),Isozymes: Current Topics in Biological and Medical Research A. R. Liss, New York, Vol. 10, p. 193.
Rahi, H., and Srivastava, P. N. (1983). Isolation and characterization of the pig endometrial arylsulfatase A.Biochem. J. 211649.
Roy, A. B. (1980). Sulphatase A: an arylsulphatase and a glycosulphatase. InSulphur in Biology (Ciba Foundation Symposium 72 Excerpta Medica, Amsterdam, p. 177.
Sarafian, T. A., Fluharty, A. L., and Kihara, H. (1984). Microheterogeneity of arylsulfatase A: treatment with hydrolytic enzymes.Biochem. Med. 3180.
Swank, R. T., Pfister, K., Miller, D., and Chapman, V. (1986). The egasyn gene affects the processing of oligosaccharides of lysosomal beta-glucuronidase in liver.Biochem. J. 240445.
Tomino, S., Paigen, K., Tulsiani, D. R. P., and Touster, O. (1985). Purification and chemical properties of mouse liver (L) beta-glucuronidase.J. Biol. Chem. 2508503.
Turner, B. M., Beratis, N. G., Turner, V. S., and Hirschhorn, K. (1974). Isozymes of alpha-L-fucosidase detectable by starch gel electrophoresis.Clin. Chim. Acta 5729.
Uehara, Y., Gasa, S., Makita, A., Sakurada, K., and Miyazaki, T. (1983). Lysosomal arylsulfatases of human leukocytes: Increment of phosphorylated B variants in chronic myelogenous leukemia.Cancer Res. 435618.
Waheed, A., and Van Etten, R. L. (1980). Chemical characterization and substrate specificity of rabbit liver arylsulfatase A.Biochim. Biophys. Acta 61492.
Waheed, A., and Van Etten, R. L. (1985). Purification of mammalian arylsulfatase A enzymes by subunit affinity chromatography.Int. J. Peptide Protein Res. 26362.
Waheed, A., Risley, J. M., and Van Etten, R. L. (1985). Structural and immunological relationships among mammalian arylsulfatase A enzymes.Comp. Biochem. Physiol. 82B855.
Watson, G., Felder, M., Rabinow, L., Moore, K., Labarca, C., Tietze, C., Vander Molen, G., Bracey, L., Brabant, M., Cai, J., and Paigen, K. (1985). Properties of rat and mouse beta-glucuronidase mRNA and cDNA, including evidence for sequence polymorphism and genetic regulation of mRNA levels.Gene 3615.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Daniel, W.L., Kahle, E.J. Arylsulfatase and beta-glucuronidase expression in green sunfish, bluegill, and their reciprocal interspecific hybrids. Biochem Genet 27, 167–181 (1989). https://doi.org/10.1007/PL00020153
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/PL00020153