A new selective medium for Burkholderia glumae was developed, which has a simpler composition and greater selectivity compared to Tsushima's S-PG medium currently used. This selective medium, designated CCNT, contains 2 g of yeast extract, 1 g of polypepton, 4 g of inositol, 10 mg of cetrimide, 10 mg of chloramphenicol, 1 mg of novobiocin, 100 mg of chlorotharonil and 18 g of agar in 1000 ml of distilled water, and is adjusted to pH 4.8. B. glumae produced a yellowish white colony with a diffusible yellow pigment on CCNT medium, which was distinguishable from other bacterial species when incubated at 41°C for 2 to 4 days. On CCNT medium, B. glumae was detected at a rate similar to that on S-PG medium in rice seeds, while other microorganisms were detected at a much lower rate.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Received 16 September 1999/ Accepted in revised form 4 January 2000
About this article
Cite this article
KAWARADANI, M., OKADA, K. & KUSAKARI, S. New Selective Medium for Isolation of Burkholderia glumae from Rice Seeds. J Gen Plant Pathol 66, 234–237 (2000). https://doi.org/10.1007/PL00012951
- Key words : Burkholderia glumae, selective medium, rice seed.