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Specificity of a Polymerase Chain Reaction Assay of a Target Sequence on the 31-Kilodalton Brucella Antigen DNA Used to Diagnose Human Brucellosis

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Abstract

 The aim of this study was to evaluate the specificity of a polymerase chain reaction assay for detecting Brucella DNA using primers specific for the amplification of a 223 bp region of the sequence encoding a 31 kDa immunogenic Brucella abortus protein (BCSP31). DNA from all Brucella strains, including type, reference, vaccine and field strains, were correctly amplified. With the exception of Ochrobactrum spp., no other amplification was detected with a broad panel of microorganisms serologically or phylogenetically related to Brucella spp. This very good degree of specificity, together with its high yield demonstrated in previous clinical studies, confirms that this polymerase chain reaction assay could be a useful tool for the diagnosis of human brucellosis.

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Authors and Affiliations

  1. Microbiology Service, "Carlos Haya" Hospital, Malaga, Spain, , , , , , ES

    M. C. Casañas

  2. Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Malaga, Spain, , , , , , ES

    M. I. Queipo-Ortuño & P. Morata

  3. Department of Microbiology, University Hospital, Faculty of Medicine, Valladolid, Spain, , , , , , ES

    A. Rodriguez-Torres & A. Orduña

  4. Infectious Diseases Unit, Department of Internal Medicine, "Carlos Haya" Hospital, Camino de Antequera s/n, 29010 Malaga, Spain e-mail: colmene@interbook.net Tel.: +34-95-2645809 Fax: +34-95-2288349, , , , , , ES

    J. D. Colmenero

Authors
  1. M. C. Casañas
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  2. M. I. Queipo-Ortuño
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  3. A. Rodriguez-Torres
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  4. A. Orduña
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  5. J. D. Colmenero
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  6. P. Morata
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Casañas, M., Queipo-Ortuño, M., Rodriguez-Torres, A. et al. Specificity of a Polymerase Chain Reaction Assay of a Target Sequence on the 31-Kilodalton Brucella Antigen DNA Used to Diagnose Human Brucellosis. EJCMID 20, 127–131 (2001). https://doi.org/10.1007/PL00011242

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  • DOI: https://doi.org/10.1007/PL00011242

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