Interactive effect of CO₂ enrichment and temperature on the photosynthesis of field-grown hinoki cypress (Chamaecyparis obtusa) branches

Abstract 

Two branches of a field-grown Chamaecy-paris obtusa tree were enclosed in chambers of an open gas exchange system for continuous CO₂ exchange measurements. One branch was subjected to ambient air (CO₂, 370 µmol mol^–1) and the other was subjected to CO₂-enriched air (800 µmol mol^–1). The CO₂ exchange rate of the branches, air temperature and photosynthetic photon flux density were recorded every 4 min by a computer during the two experimental periods of July 1994 to June 1995 (experiment 1) and April 1996 to August 1997 (experiment 2). The response of CO₂ gas exchange rate to light changed with the seasonal temperature. The highest saturated rate of net photosynthesis on a leaf area basis was observed in May and October in both CO₂ treatments when the mean daytime temperature was about 18–19°C. This temperature was almost equal to the yearly mean daytime temperature. Above and below this temperature, the saturated net photosynthesis rate decreased. The net photosynthesis rate was usually higher in the elevated CO₂ treatment. The ratio of monthly net photosynthesis rate in elevated CO₂ to that in ambient CO₂ was linearly related to the monthly mean daytime temperature. This ratio increased by 3.3% for each 1°C increase in the monthly mean daytime temperature; the highest ratio of 1.8 occurred in August. When the ratio was 1.0, the temperature was about 5–6°C, which was close to the mean daytime temperature of the coldest month. Elevated CO₂ increased per unit area net photosynthesis by 38.5% and 43.7% in experiments 1 and 2, respectively.