Internal oxygen levels decrease during the growing season and with increasing stem height

Abstract 

The oxygen levels in the sapwood of Norway spruce [Picea abies Karst. (L.)] trees were measured by GC-MS (gas chromatography – mass spectrometry) as stem growth progressed during the growing season. The measurements were made on 30-year-old, 13–15 m high, trees growing on three plots which were irrigated, left undisturbed or subjected to drought. The oxygen measurements were done at 1.5, 4 and 8 m above the ground. The internal oxygen levels dropped to about 5% of the level in air in irrigated trees, 1–3% in control trees and to below 1% in trees subject to drought between mid-July and mid-August. In the trees subjected to drought there where no differences in internal oxygen concentrations between the positions on the tree during the growing season except for a faster increase back to ambient concentration occurring in late August in the 1.5 m position. In trees growing on the two other plots the internal oxygen concentration was higher near the ground than at 4 or 8 m above ground during July and August. At all other dates there were no differences between the positions. The results indicate that there is an oxygen gradient with levels decreasing acropetally, giving indirect support to the hypothesis that oxygen is supplied to the growing stem in the transpiration stream.