Abstract
Extracellular polymeric substances (EPS) were removed by mechanical (high-speed centrifugation) and chemical (EDTA treatment) methods. The number of attached microorganisms decreased from 33.0 × 107 CFU/cm2 to 17.5 × 107 CFU/cm2 and 12.5 × 107 CFU/cm2, respectively. When the activated sludge was treated with polysaccharide-oxidizing agent (sodium meta periodate) and protease (pronase E), the number of attached microorganisms decreased to 41% and 43.5%, respectively. Transmission electron microphotographs showed that polysaccharide-oxidizing agent and protease treatment caused the removal of filamentous extracellular structures.
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Received: 18 January 1999
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Park, Y., Kim, D., Park, T. et al. Effect of extracellular polymeric substances (EPS) on the attachment of activated sludge. Bioprocess Engineering 22, 1–3 (2000). https://doi.org/10.1007/PL00009092
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DOI: https://doi.org/10.1007/PL00009092