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Effects of agitation and aeration on the production of extracellular glucose oxidase from a recombinant Saccharomycescerevisiae

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Abstract

A recombinant strain of Saccharomyces cerevisiae, GAL-GO2, was developed to facilitate the production of extracellular glucose oxidase (GOD). The recombinant strain secreted 85% (8.7 U/ml) of the total GOD (10.3 U/ml) produced in shake flask culture. For further enhancement of GOD production, optimization of the speed of agitation and the rate of aeration in a stirred tank fermentor was carried out. Response surface methodology with appropriate statistical experimental design was employed for this purpose. The maximal level of extracellular GOD was achieved when the speed of agitation and the rate of aeration were 420 rpm and 0.25 vvm, respectively. The enzyme production was increased by 74% compared to the level obtained under unoptimized conditions.

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Authors and Affiliations

  1. Biopilot Plant, Korea Research Institute of Bioscience and Biotechnology, KIST, P.O. Box 115, Yusong, Taejon 305-600, Korea, e-mail: ypark@kribb4680.kribb.re.kr, , , , , , KR

    A. Kapat, J. K. Jung & Y. H. Park

  2. Biotechnology group, Doosan Training & Technology Center, 39-3, Songbok-Ri, Suji-Myon, Yong-In, Kyonggi-Do 449-840, Korea, , , , , , KR

    S. Y. Hong & H. K. Choi

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  1. A. Kapat
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  2. J. K. Jung
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  3. Y. H. Park
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  4. S. Y. Hong
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  5. H. K. Choi
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Received: 29 May 1997

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Kapat, A., Jung, J., Park, Y. et al. Effects of agitation and aeration on the production of extracellular glucose oxidase from a recombinant Saccharomycescerevisiae . Bioprocess Engineering 18, 347–351 (1998). https://doi.org/10.1007/PL00008994

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  • DOI: https://doi.org/10.1007/PL00008994

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