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The effect of oxygen transfer on the activity of encapsulated whole cell β-galactosidase

Abstract

The effect of oxygen transfer on the production of immobilized whole cell β-galactosidase has been evaluated. The encapsulated whole cell β-galactosidase was prepared by combining cell encapsulation and culture into one-step. Escherichia coli was encapsulated and cultured in the growth and production media to accumulate β-galactosidase in itself. Sunflower seed oil was coimmobilized to increase the oxygen transfer rate through the capsule membrane. The oxygen transfer rate increased 63 percent and the activity of β-galactosidase increased by 10 percent. The activity of encapsulated β-galactosidase obtained in the concentric air lift reactor was 86 percent higher than that in the shaking incubator. In the concentric air lift reactor, the accumulation of encapsulated whole cell β-galactosidase was primarily dependent on the capsule velocity. While the accumulation of specific β-galactosidase in the capsule increased with volumetric oxygen transfer coefficient, the cell biomass accumulated in the capsule decreased.

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Received: 9 December 1996

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Park, J., Jeong, G. & Chang, H. The effect of oxygen transfer on the activity of encapsulated whole cell β-galactosidase. Bioprocess Engineering 17, 197–202 (1997). https://doi.org/10.1007/PL00008964

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  • DOI: https://doi.org/10.1007/PL00008964

Keywords

  • Biomass
  • Escherichia Coli
  • Transfer Coefficient
  • Encapsulation
  • Production Medium