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Structure and regulation of the dnaA promoter region in three Streptomyces species

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Abstract

 The regulatory region of the Streptomyces dnaA gene comprises a single promoter and two DnaA boxes that are located upstream of the promoter. Comparative analysis of the dnaA promoter region from S. chrysomallus, S. lividans and S. reticuli revealed that the location, spacing and orientation of the DnaA boxes are conserved. In vitro studies demonstrated that efficient binding of the Streptomyces DnaA protein to DNA requires the presence of two DnaA boxes. In vivo analysis of dnaA promoter mutants deleted for one or both DnaA boxes indicated that the dnaA gene is autoregulated. However, the degree of derepression observed is relatively modest.

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Authors and Affiliations

  1. Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, ul. Weigla 12, 53-114 Wroclaw, Poland E-mail: zakrzew@immuno.iitd.pan.wroc.pl Tel.: +48-71-3732274; Fax: +48-71-3732587, , , , , , PL

    D. Jakimowicz, J. Majka, B. Lis & J. Zakrzewska-Czerwinska

  2. Max-Planck-Institut für molekulare Genetik, Ihnestrasse 73, 14195 Berlin-Dahlem, Germany, , , , , , DE

    W. Messer

  3. Department of Molecular Biology, University of Gdansk, Kladki 24, 80-822 Gdansk, Poland, , , , , , PL

    G. Konopa & G. Wegrzyn

  4. Fachbereich Biologie/Chemie, Universität Osnabrück, Barbarastrasse 11, 49069 Osnabrück, Germany, , , , , , DE

    H. Schrempf

Authors
  1. D. Jakimowicz
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  2. J. Majka
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  3. B. Lis
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  4. G. Konopa
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  5. G. Wegrzyn
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  6. W. Messer
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  7. H. Schrempf
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  8. J. Zakrzewska-Czerwinska
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Received: 12 July 1999 / Accepted: 19 October 1999

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Jakimowicz, D., Majka, J., Lis, B. et al. Structure and regulation of the dnaA promoter region in three Streptomyces species. Mol Gen Genet 262, 1093–1102 (2000). https://doi.org/10.1007/PL00008652

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  • DOI: https://doi.org/10.1007/PL00008652

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