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Chromosoma

, Volume 106, Issue 8, pp 513–519 | Cite as

A repetitive DNA sequence common to the different B chromosomes of the genus Brachycome

  • Andreas Houben
  • Carolyn R. Leach
  • Dawn Verlin
  • Ruth Rofe
  • Jeremy N. Timmis

Abstract. 

Dot-like micro B chromosomes of Brachycome dichromosomatica were analysed for their sequence composition. Southern hybridization patterns of a total micro B probe to genomic DNA from plants with and without micro Bs demonstrated that the micro Bs shared sequences with the A chromosomes. In addition to telomere, rDNA and common A and B chromosome sequences, a new B-specific, highly methylated tandem repeat (Bdm29) was detected. After in situ hybridization with Bdm29 the entire micro B chromosome was labelled and clustering of the condensed micro Bs could be observed at interphase. A high number of Bdm29-like sequences were also found in the larger B chromosomes of B. dichromosomatica and in other Bs within the genus Brachycome.

Keywords

Methylated Tandem Repeat Southern Hybridization Hybridization Pattern Sequence Composition 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Andreas Houben
    • 1
  • Carolyn R. Leach
    • 1
  • Dawn Verlin
    • 1
  • Ruth Rofe
    • 1
  • Jeremy N. Timmis
    • 1
  1. 1.Department of Genetics, The University of Adelaide, SA 5005, AustraliaAU

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