Abstract.
Pseudomonas saccharophila NRRL B-628 is capable of utilizing agricultural lipids for growth. The organism exhibited good growth with triacylglycerol substrates that contained saturated fatty acyl moieties such as coconut oil (CO; C10–12 fatty acids) and tallow (T; C16–18 fatty acids). Electron micrographs of the triacylglycerol-grown cells showed the presence of intracellular granules indicative of poly(β-hydroxyalkanoate) (PHA) production. Cells grown in a 250-ml CO-containing medium produced ca. 0.2 g of medium-chain-length (mcl)-PHA. Gas chromatographic analysis showed that β-hydroxyoctanoic acid (30%), β-hydroxydecanoic acid (40%), and β-hydroxydodecanoic acid (16%) were the major monomer repeat-units of the CO-derived polymer. The estimated mean molecular mass of the CO-derived mcl-PHA as determined by gel permeation chromatography was 13.1 × 104 g/mol with a polydispersity of 3.16.
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Received: 15 August 1998 / Accepted: 25 September 1998
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Solaiman, D., Ashby, R. & Foglia, T. Medium-Chain-Length Poly(β-Hydroxyalkanoate) Synthesis from Triacylglycerols by Pseudomonas saccharophila . Curr Microbiol 38, 151–154 (1999). https://doi.org/10.1007/PL00006779
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DOI: https://doi.org/10.1007/PL00006779