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Journal of Molecular Evolution

, Volume 48, Issue 6, pp 740–749 | Cite as

Primary and Secondary Structure of the Small-Subunit Ribosomal RNA of the Naked, Marine Amoeba Vannella anglica: Phylogenetic Implications

  • Gary P.  Sims
  • Andrew  Rogerson
  • Robert  Aitken

Abstract.

The primary and secondary structure of the small-subunit ribosomal RNA (ssrRNA) gene from the naked, marine amoeba, Vannella anglica (subclass Gymnamoebia), was determined. The ssrRNA is 1962 nucleotides in length, with a low G+C content of 37.1%. The ssrRNA is composed of several uncommon secondary structure features including helix E8-1, which may be a useful target for rRNA probes for the direct identification of isolates in mixed culture. Phylogenetic analysis of sequence data showed that V. anglica branched prior to the rapid diversification of the eukaryotes. It did not associate with the other naked, lobose amoebae represented by Acanthamoeba and Hartmannella, indicating that Vannella represents a separate amoeboid lineage and the subclass Gymnamoebia is polyphyletic.

Key words: Small-subunit ribosomal RNA — Molecular phylogeny — Rhizopoda — Amoeba — Gymnamoebae — Secondary structure — Crown taxa — G+C content 

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Copyright information

© Springer-Verlag New York Inc. 1999

Authors and Affiliations

  • Gary P.  Sims
    • 1
  • Andrew  Rogerson
    • 1
  • Robert  Aitken
    • 2
  1. 1.University Marine Biological Station Millport, Isle of Cumbrae, KA28 OEG ScotlandGB
  2. 2.Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, ScotlandGB

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