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Journal of Molecular Evolution

, Volume 48, Issue 6, pp 717–722 | Cite as

Intracellular Bacterial Symbionts of Aphids Possess Many Genomic Copies per Bacterium

  • Kanako  Komaki
  • Hajime  Ishikawa

Abstract.

Although Buchnera, the endosymbiotic bacteria of aphids, are close relatives of Escherichia coli, their genome size is only a seventh that of E. coli. In this study, we estimated the genomic copy number of Buchnera by dot-blot hybridization and fluorimetry using a video-intensified microscope photon-counting system and obtained convincing evidence that each cell of these bacteria contains an average of 120 genomic copies. Thus, the Buchnera symbiont, with many copies of a small-sized genome, is reminiscent of cell organelles such as mitochondria and chloroplasts.

Key words:Buchnera— Pea aphid — Symbiosis — Genomic copy number — Dot-blot analysis — Microscope photon-counting system — Polyploidy 

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Copyright information

© Springer-Verlag New York Inc. 1999

Authors and Affiliations

  • Kanako  Komaki
    • 1
  • Hajime  Ishikawa
    • 1
  1. 1.Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, JapanJP

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