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Journal of Molecular Evolution

, Volume 46, Issue 3, pp 299–306 | Cite as

Ribosomal External and Internal Transcribed Spacers: Combined Use in the Phylogenetic Analysis of Medicago (Leguminosae)

  • Gilles  Bena
  • Marie-France  Jubier
  • Isabelle  Olivieri
  • Bernard  Lejeune

Abstract.

We have estimated the potential phylogenetic utility of the ribosomal external transcribed spacer (ETS) from the nuclear ribosomal region. The ETS was sequenced from 13 annual Medicago (Fabaceae) species upstream a highly conserved motive which was found among many different organisms. In the genus Medicago, the ETS was found to evolve 1.5 times faster than the internal transcribed spacer and to be 1.5 times more informative. Reduced ribosomal maturation process constraints on ETS are proposed to explain the different evolutionary rates between the two spacers. Maximal phylogenetic resolution and support was obtained when the two spacers were analyzed together. No incongruence between the two spacers was found and ETS appears to be a valuable source of information for solidifying ITS plant phylogeny. The phylogeny obtained in Medicago suggests that none of the three subsections included in the study is monophyletic.

Key words: Phylogeny — ETS — ITS —Medicago 

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Copyright information

© Springer-Verlag New York Inc. 1998

Authors and Affiliations

  • Gilles  Bena
    • 1
  • Marie-France  Jubier
    • 1
  • Isabelle  Olivieri
    • 2
  • Bernard  Lejeune
    • 1
  1. 1.Institut de Biotechnologie des Plantes, Université Paris-Sud, Bâtiment 630, F-91405 Orsay Cedex FranceFR
  2. 2.Laboratoire de Ressources Génétiques et d'Amélioration des Luzernes Méditerranéennes, INRA Montpellier-Domaine de Melgueil, 34130 Mauguio FranceFR

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