Abstract
The contractile and electrical properties of the mouse diaphragm during endotoxemia were studied, and the possible role of nitric oxide (NO) on these changes was investigated. The mice were injected intraperitoneally with E. coli. lipopolysaccharide (endotoxin, LPS) at various times before isolation of the diaphragm to induce endotoxemia. It was observed that direct twitch tension was slightly increased, and that there was a significant increase in tetanic tension when compared with controls. The potentiation of direct twitch tension induced by a Cl–-channel blocker (9-anthracene carboxylic acid), but not the potentiation by a Na+-channel activator (veratridine) or by K+-channel blockers (uranyl ion, 4-aminopyridine and tetraethylammonium ion), was attenuated in the diaphragm of LPS-treated mice. Moreover, the resting membrane potential was significantly reduced and the membrane input resistance was significantly increased, largely due to a decrease in Cl–-conductance. However, the membrane K+-conductance remained unaltered. These results imply that the sarcolemmal Cl–-channel is markedly affected in the mouse diaphragm during endotoxemia. These changes of contractile and electrical characteristics of the mouse diaphragm during endotoxemia could be reversed by treatment with dexamethasone and NG-nitro-l-arginine (NO synthase inhibitors). On the other hand, in in vitro studies, LPS (20 μg/ml), by itself, applied directly to the diaphragm, did not alter the muscle contractions or the membrane potentials. A NO donor, added to the diaphragm bath, increased the tetanus/twitch ratio and induced a transient depolarization. All of these findings suggest that LPS may, at least in part, affect the sarcolemmal electrical properties and muscle contractions during endotoxemia through the l-arginine:NO pathway.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 3 February 1997 / Accepted: 16 May 1997
Rights and permissions
About this article
Cite this article
Liu, S., Lai, J., Lin, R. et al. Involvement of nitric oxide in the in vivo effects of lipopolysaccharide on the contractile and electrical properties of mouse diaphragm. Naunyn-Schmiedeberg's Arch Pharmacol 356, 500–504 (1997). https://doi.org/10.1007/PL00005083
Issue Date:
DOI: https://doi.org/10.1007/PL00005083