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Insectes Sociaux

, Volume 48, Issue 3, pp 266–272 | Cite as

Genetic differentiation of the honey bee (Apis cerana) in Thailand revealed by polymorphism of a large subunit of mitochondrial ribosomal DNA

  • S. Sittipraneed
  • D. Sihanuntavong
  • S. Klinbunga

Summary:

The large subunit of the ribosomal (lr) RNA gene of Thai Apis cerana was amplified and sequenced. The average sequence divergence among geographically diverse bees was 1.13% (0.15% - 1.70%, n = 8). Based on these nucleotide sequences, genetic differentiation analysis of A. cerana was simplified to a RFLP based on Dra I. Four haplotypes of digested lrRNA gene were observed. Haplotype A was found in north, north-east and the central region (collectively called the north-to-central sample) whereas haplotype B was restricted to specimens from peninsular Thailand, Phuket and Samui Islands (collectively called the southern sample). Haplotype C was found in 47.06% of A. cerana originating from Samui Island but was not found in other geographic samples. Geographic heterogeneity analysis and F ST statistics indicated the existence of population differentiation of A. cerana in Thailand (P < 0.0001). Analysis of molecular variance (AMOVA) also illustrated significant genetic differences between bees from the north-to-central region (A), peninsular Thailand and Phuket (B) and Samui Island (C) (P < 0.0001).

Key words: Honey bees, Apis cerana, PCR-RFLP, genetic diversity, population differentiation. 

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Copyright information

© Birkhäuser Verlag, 2001

Authors and Affiliations

  • S. Sittipraneed
    • 1
  • D. Sihanuntavong
    • 1
  • S. Klinbunga
    • 2
  1. 1.Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand, e-mail: ssiripor@netserv.chula.ac.th TH
  2. 2.National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Bangkok 10400, Thailand TH

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