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Cellular and Molecular Life Sciences CMLS

, Volume 58, Issue 2, pp 234–243 | Cite as

Crystallization of RNA

  • S. R. Holbrook*
  • E. L. Holbrook
  • H. E. Walukiewicz

Abstract.

Even as the number of RNA structures determined and under study multiplies, the critical step in X-ray diffraction analysis, growth of single well-ordered crystals, remains at the boundary between art and science. Recent advances in methods of RNA synthesis, purification, and characterization, as well as empirical and technical improvements in crystallization techniques, the development of cryo-crystallography, and the wider availability of bright, tunable, X-rays from synchrotron sources are improving the chances of obtaining RNA crystals suitable for X-ray structural analysis. In this review, we summarize the current status of the design, preparation, purification, and analysis of RNA for crystallization and describe the latest approaches to obtaining diffraction-quality crystals.

Key words. RNA crystallization; X-ray crystallography; RNA crystal structure; sparse matrix crystallization; X-ray diffraction. 

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Copyright information

© Birkhäuser Verlag Basel, 2001

Authors and Affiliations

  • S. R. Holbrook*
    • 1
  • E. L. Holbrook
    • 1
  • H. E. Walukiewicz
    • 1
  1. 1.Structural Biology Department, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley (California 94720, USA), Fax +1 510 486 6059, e-mail: SRHolbrook@lbl.gov US

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