Abstract.
E-selectin, exclusively expressed on activated endothelial cells, is a potential target for site-directed delivery of agents. We and others have shown that sialyl Lewisx-liposomes (sLex-liposomes) are recognized by E-selectin. We now report an approach employing sLex-liposomes to deliver antisense oligonucleotides (AS-ODNs) directed against the adhesion molecule ICAM-1 to activated vascular endothelial cells. ICAM-1 expression was analyzed at the protein level by immunofluorescence and a cell surface ELISA, and at the RNA level by RT-PCR. We have investigated two different AS-ODNs complementary to the 3′ untranslated region and the AUG translation initiation codon of ICAM-1 mRNA. Both inhibited protein expression, but did not influence the mRNA level, pointing to a hybridization of AS-ODNs with the mRNA in the cytoplasm. Our results demonstrate the feasibility of a novel approach for the delivery of agents to activated endothelial cells by glycoliposomes targeted to E-selectin.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received 16 October 2000; revised 29 November 2000; accepted 29 November 2000
Rights and permissions
About this article
Cite this article
Stahn*, R., Grittner, C., Zeisig, R. et al. Sialyl Lewisx-liposomes as vehicles for site-directed, E-selectin-mediated drug transfer into activated endothelial cells . CMLS, Cell. Mol. Life Sci. 58, 141–147 (2001). https://doi.org/10.1007/PL00000774
Issue Date:
DOI: https://doi.org/10.1007/PL00000774