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The impact of inflammation to the antioxidant defense parameters in AMD patients

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Abstract

Background and aims: Oxidative stress and inflammation are postulated to be involved in the pathogenesis of the age-related macular degeneration (AMD) although the mechanism linking the oxidation and inflammation is still unknown. The aim of this study was the analysis of the antioxidant capacity measured by levels of the antioxidant enzymes: superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR) and total antioxidant status (TAS) along with the inflammatory markers such as Creactive protein (CRP), interleukin-6 (IL-6) and fibrinogen in AMD patients in order to analyze the relationship of the inflammatory markers with the antioxidant parameters and their association with AMD. Methods: The cross-sectional study, carried out in the University clinical setting, included 84 patients with the age-related macular degeneration, aged 71.25±7.14 years and 84 aged-matched control subjects (CG). Results: Statistical analysis revealed significantly lower GR (p=0.007) and TAS (p<0.000) values in the group of AMD patients compared to the controls. Logistic regression analysis showed that higher values of inflammatory markers (CRP>3 mg/L, IL>4.9 pg/mL, fibrinogen>3.8 g/L) and lower values of antioxidative parameters (SOD<900 U/gHb, GR<55 U/L and TAS<1.15 mmol/L) were significantly associated with AMD (ORCRP: 1.29, 95% CI 0.54-3.12, p<0.05; ORIL-6: 3.53, 95% CI 1.16–10.75, p=0.024; ORFIB: 3.06, 95% CI 1.78–7.92, p=0.019; ORSOD: 2.39, 95% CI 0.78–7.35, p<0.05; ORGR: 4.04, 95% CI 1.28–12.73, p=0.013; ORTAS: 2.9, 95% CI 1.4–6.3, p=0.032). Conclusions: Based on the results obtained, it may be concluded that the antioxidant defense system was significantly reduced in patients with AMD and the probability to develop AMD was higher in older individuals with lower values of antioxidant parameters and higher values of inflammatory markers.

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Authors and Affiliations

  1. Institute of Medical Biochemistry, Clinical Center of Serbia, School of Pharmacy, University of Belgrade, Višegradska 26, 11000, Belgrade, Serbia

    Emina Čolak M.Sc. & Nada Majkić-Singh

  2. Institute of Ophthalmology, Medical Retina Department, Clinical Center of Serbia, School of Medicine, University of Belgrade, Belgrade, Serbia

    Lepša Žorić, Aleksandra Radosavljević & Natalija Kosanović-Jaković

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  1. Emina Čolak M.Sc.
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  2. Nada Majkić-Singh
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  4. Aleksandra Radosavljević
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  5. Natalija Kosanović-Jaković
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Correspondence to Emina Čolak M.Sc..

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Čolak, E., Majkić-Singh, N., Žorić, L. et al. The impact of inflammation to the antioxidant defense parameters in AMD patients. Aging Clin Exp Res 24, 588–594 (2012). https://doi.org/10.1007/BF03654844

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