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Intraarterial Protamine Sulfate Reduces the Magnitude of Streaming Potentials in Living Canine Tibia

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Abstract

Using previously described techniques, transcortical streaming potentials were measured at two middiaphyseal sites on one tibia of each of nine anesthetized canines during sinusoidal bending (~0 to −200 μє periosteal surface strain) at 2 Hz. Measurements were made for 60 minutes prior to and up to 180 minutes following bolus injection of protamine sulfate (42-126 mg/kg) dissolved in Hanks Balanced Salt Solution, directly into the femoral artery without interrupting circulation. Shortly after injection, the protamine sulfate caused a clear reduction in the magnitude of streaming potentials. Subsequent injections of additional protamine sulfate resulted in further reductions, and in several instances, voltage sign reversals. This study represents the first observation that circulating proteins may alter electromechanical transduction in living bone, and suggests the possibility that specific agents, which are known to affect bone remodeling, may do so, in part, by altering these endogenous electrical potentials.

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Acknowledgments

This work was funded by NIH ROI AR39574, the Walter Scott Fund, the NY State DOH, and supported by the VAMC at Castle Point, NY, where the work was performed. We thank Dr. Bok Lee and Ms Brenda Signor for their assistance with this project.

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Authors and Affiliations

  1. Orthopaedic Engineering and Research Center Helen Hayes Hospital (OERC), Route 9W, West Haverstraw, New York, 10993, USA

    Mark W. Otter, Dadong D. Wu, William A. Bieber & George B. Van Cochran

  2. VA Medical Center, Castle Point, New York, 12511, USA

    Mark W. Otter & George B. Van Cochran

  3. The Department of Orthopaedic Surgery, Columbia University, New York, 10032, USA

    George B. Van Cochran

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  1. Mark W. Otter
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  2. Dadong D. Wu
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  3. William A. Bieber
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  4. George B. Van Cochran
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Otter, M.W., Wu, D.D., Bieber, W.A. et al. Intraarterial Protamine Sulfate Reduces the Magnitude of Streaming Potentials in Living Canine Tibia. Calcif Tissue Int 53, 411–415 (1993). https://doi.org/10.1007/BF03549784

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  • DOI: https://doi.org/10.1007/BF03549784

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