Summary
The objective of this study was to determine the potential of the chlorophyll (Chl) fluorescence technique to assess the behaviour of wheat varieties under drought and heat in the field. Seedlings of Flamura 85, a drought and heat resistant wheat cultivar, were exposed to 35°C for 2 up to 72 h. Net photosynthesis decreased significantly after 48 and 72 h of high temperature exposure, whereas the Chl fluorescence ratios, the Rfd-values at 690 and 735 nm, and the stress adaptation index Ap decreased much faster, after 4 h already, demonstrating that Chl fluorescence is a useful trait in early detection of stress in wheat cultivars. The best differentiation of controls from heat-exposed plants for the Chl fluorescence indices (Rfd-values and Ap) was obtained at 48 h. Ten winter wheat varieties were investigated for the ratios of the variable Chl fluorescence, Rfd-values at 690 and 735 nm as vitality indices, and the stress adaptation index, Ap, in primary leaves after seedlings’ exposure to 35°C for 48 h. Chl fluorescence parameters significantly decreased in all genotypes, but to a higher extent in those which are drought and heat sensitive according to field evaluations. The better performing varieties under stress conditions in the field also showed a smaller decrease of the Chl fluorescence indices. When plotting the differences between Ap at normál and high temperature against the mean visual score for the leaves’ vitality in the field during the anthesis-grain filling period, a significant high correlation was found (r = 0.78**).
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Abbreviations
- Pn:
-
net photosynthesis
- Chl:
-
chlorophyll
- gs:
-
stomatal conductance
- ci:
-
internal CO2 concentration
- Rfd:
-
ratio of variable Chl fluorescence (fluorescence decrease ratio)
- Ap:
-
stressadaptation index
- a+b:
-
chlorophyll a and b content
- x+c:
-
total carotenoids content
References
Babani, F and Lichtenthaler, H.K. 1996. Light-induced and age-dependent development of chloroplasts in etiolated barley leaves as visualized by determination of photosynthetic CO2 assimilation rates and different kinds of chiorophyll fluorescence ratios. J. Plant Physio. 148: 555–566.
Balota, M. 1997. Cercetari fiziologice privitoare la rezistenta graului hexaploid (T. aestivum L.) la conditii de stres hidric si termic. Ph.D. Thesis. University of Bucharest, Romania.
Buschmann, C., Richter, P. and Lichtenthaler, H.K. 1996. Application of the Karlsruhe CCD- OMA LID AR- fluorosensor in stress detection of plants. J. Plant Physiol. 148: 548–554.
Kautsky, H. and Hirsch, A. 1931. Neue Versuche zur Kohlenstoffassimilation. Naturwiss. 19: 964.
Krause, G.H. and Weiss, E. 1991. Chiorophyll fluorescence and photosynthesis: The basics. Annu. Rev. Plant Physiol. Plant Mol. Biol. 42: 313–349.
Lang, M., Lichtenthaler, H.K., Sowinska, M., Heisel, F., Miehé, J.A. and Tomasini, F. 1996. Fluorescence imaging of water and temperature stress in plant leaves. J. Plant Physiol. 148: 613–621.
Lichtenthaler, H.K. 1987a. Chiorophyll fluorescence signatures of leaves during the autumnal chiorophyll breekdown. J. Plant Physiol. 131: 101–110.
Lichtenthaler, H.K. 1987b. Chlorophylls and carotenoids, the pigments of photosynthetic biomembranes. Methods in Enzymol. 148: 350–382.
Lichtenthaler, H.K. 1990. Applications of chiorophyll fluorescence in stress physiology. In: Applications of Remote Sensing in Agriculture. Steven M. and Clarke J.A. (eds.). Butterworths Scientific Ltd., London, pp. 287–305.
Lichtenthaler, H.K. 1992. The Kautsky Effect: 60 years of chiorophyll fluorescence induction kinetics. Photosynthetica 27, pp 45–55.
Lichtenthaler, H.K. and Rinderle, U. 1988. Chiorophyll fluorescence signatures of healthy and damaged spruce trees. PEF-Bericht KfK-PE. 35: 185–190.
Lichtenthaler, H.K. and Miehé, J.A. 1997. Fluorescence imaging as diagnostic tool for plant stress. Trends in Plant Science. 2: 316–320.
Lichtenthaler, H.K., Buschmann, C, Rinderle, U. and Schmuck, G. 1986. Application of chiorophyll fluorescence in ecophysiology. Radiat Environ. Biophys. 25: 297–308.
Santarius, K. A. and Muller, M. 1979. Investigations on heat resistance in spinach leaves. Plant. 146: 529–538.
Saulescu N.N., G. Ittu, M. Balota, M. Ittu & P. Mustate. 1998. Breeding wheat for lodging resistance, earliness and tolerance to abiotic stresses. In: Wheat: Prospects for Global Improvement, Braun J.H. et al. (Eds.), Kluwer Academic Publishers, pp 181–188.
Schweiger, J., Lang, M. and Lichtenthaler, H.K. 1996. Differences in fluorescence excitation spectra of leaves between stressed and non-stressed plants. J. Plant Physiol. 148: 536–547.
Sokal, R.R. and F.J. Rohl. 1973. “Single classification analysis of variance” and “Two-way analysis of variance”. In: Introduction to Biostatistics, Kennedy D. and Park R.B. (Eds.), W.H. Freeman, San Francisco, pp. 161–179 and 185–198.
Strasser, R., Schwarz, B. and Bucher, J. 1987. Simultane Messung der Chlorophyllfluores- zenz-Kinetik bei verschiedenen Wellenlangen als rasches Verfahren zur Friihdiagnose von Immissionsbelastungen an Waldbäumen. Europ. Forest Patholog. 17: 149–157.
Thebud, R. and Santarius, K. 1982. Effects of high temperature stress on various biomem- branes of leaf cells in situ and in vitro. Plant Physiol. 70: 200–205.
Tuba Z., Lichtenthaler H.K., Csintalan Z., Nagy Z., Szente K. 1994. Reconstitution of chlorophylls and photosynthetic CO2 assimilation upon rehydration of the desiccated poikilochlorophyllous plant Xerophyta scabrida. Plant. 192: 414–420.
Xu Q., Paulsen AQ., Guikema J.A., Paulsen G.M. 1995. Functional and ultrastructural injury to photosynthesis in wheat by high temperature during maturation. Environm. Experiment. Botany. 35: 43–54.
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Balota, M., Lichtenthaler, H.K. Red Chlorophyll Fluorescence as an Ecophysiological Method to Assess the Behaviour of Wheat Genotypes Under Drought and Heat. CEREAL RESEARCH COMMUNICATIONS 27, 179–187 (1999). https://doi.org/10.1007/BF03543935
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DOI: https://doi.org/10.1007/BF03543935