Summary
Four induction and six regeneration media were used to obtain haploid plants from three triticale genotypes and their F1 hybrids. The induction media which contained 2 mg/1 2,4-D (2,4-Dichlorophenoxyacetic acid) and 50 g/1 sucrose plus 50 g/1 maltose or 100 g/1 maltose respectively were the best at inducing embryogenesis, although strong genotype differences were found. RG0 medium without hormones was found to be the most successful with respect to plantlet regeneration. No differences were observed between regeneration media with respect to the ratio of green to albino plants produced. When combined use was made of the best induction and regeneration media, the number of haploid green plants per 100 anthers plated ranged between 2.42 and 15.36. It might be concluded that a successful androgenetic response can be obtained by combining SM or M induction and RG0 regeneration media in the breeding of triticale.
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References
Atanassov, A., N. Nagorska, P. Boyadjiev, and D. Djilianov, 1995. In vitro production of haploid plants. World J. Microbiol. Biotechnol. 11:400–408
Ball, S.T., H. Zhou and C.F. Konzak, 1992. Effects of sucrose concentration and its functional relationship on the haploid yield components of wheat. Crop. Sci. 32:149–154
Barceló, P., A. Cabrera, C. Hagel and H. Lörz, 1994. Production of doubled-haploid plants from tritordeum anther culture. Theor. Appl. Genet. 87:741–745
Bernard, S., 1980. In vitro androgenesis in hexaploid triticale: determination of physical conditions increasing embryo and green plant production. Z. Pflanzenzüchtg 85:308–321
Brisive, E.A., A. Olesen and S.B. Andersen, 1997. Characterization of anther culture-derived cell suspensions exclusively regenerating green plantlets in wheat (Triticum aestivum L.) Euphytica 93:321–329
Bullock, W.P., P.S. Baenziger, G.W. Sheaffer and P.J. Bottino, 1982. Anther culture of wheat (T. aestivum L.) F1 and their reciprocal crosses. Theor. Appl. Genet. 62:155–159
Chu, C.C., 1978. The N6 medium and its application to anther culture of cereal crops. In: Proc. Symp. Plant Tissue Culture, pp 43–50. Science Press, Beijing
Chu, C.C., R.D. Hill and A.L. Brule-Babel, 1990. High frequency of pollen embryo formation and plant regeneration in Triticum aestivum L. on monosaccharides-containing media. Plant Sci. 66:255–262
Chuang, C.C., T.W. Ouyang, H. Chia, S.M. Chou and C.K. Ching, 1978. A set of potato media for wheat anther culture. In: Proc. Symp. Plant Tissue Culture, pp 51–56. Science Press, Beijing
Deimling, S., T. Flehinghaus, M. Boh and H.H. Geiger, 1992. Sugary aspects of androgenesis alterations of carbohydrates during haploid induction i gramineous species. In: Abstracts XIIIth EUCARPIA Congress, Angers, July 6–11. pp 153–154
Faurie, C, 1992. Androgenèse in vitro chez le triticale: Recherche de conditions environnementales optimales, Influence du niveau de génératin des plantes-mères sur la réponse à la méthode et sur les caractéristiques cytologiques et agronomiques des plantes androgénétiques. Thèse. Université Blaise Pascal. Clermont-Fd.
Flehinghaus, T., S. Deimling and H.H. Geiger, 1991. Methodical improvements in rye anther culture. Plant Cell Rep. 10:397–400
Flehinghaus-Roux, T., S. Deimling and H.H. Geiger, 1995. Anther-culture ability in Secale cereale L. Plant Breeding 114:259–261
Ghaemim M., A. Sarrafi and G. Alibert, 1993. Influence of genotype and culture conditions on the production of embryos from anthers of tetraploid wheat (Triticum turgidum). Euphytica 81–85
Ghaemi M., A. Sarrafi and G. Alibert, 1995. Influence of genotype, media composition, cold pretreatment and their interactions on androgenesis in durum wheat (Triticum turgidum) Cer. Res. Comm. 23:215–222
González, J.M., I. Hernández and N. Jouve, 1997. Analysis of anther culture response in hexaploid triticale. Plant Breeding 116:302–304
Hassawi, D.S. and G.H. Liang, 1990. Effect of cultivar, incubation temperature and stage of microspore development on anther culture in wheat and triticale. Plant Breeding 105:40–45
Henry, Y. and J. De Buyser, 1985. Effect of the 1B/1R translocation on anther culture ability in wheat (Triticum aestivum L.) Plant Cell Rep. 4:307–310
Hunter C.P., 1987. European Patent Application by Shell International Reseach Maatschappij B.V. No 87200773.7
Jones, A.M. and J.F. Petolino. 1987. Effects of donor plant genotype and growth environment on anther culture of soft-red winter wheat (Triticum aestivum L) Plant Cell Tissue and Organ Culture 8:215–223
Marciniak, K., Z. Banaszak and M. Wedzony, 1998. Effect of genotype, medium and sugar on triticale (×Triticosecale Wittm.) anther culture response. Cereal Res. Comm. 26:145–151
Miller, C.O., 1963. Kinetin and kinetin-like compounds. In: Moderne Methoden der Pflanzen-analyse. Eds. H.F. Lenskens and M.C. Tracey 6:193–202. Springer-Verlag, Berlin
Moieni A., and A. Sarrafi, 1995. Genetic analysis for haploid regeneration responses of hexaploid wheat anther culture. Plant Breeding 114:247–249
Moieni A., K. Lokos-Toth and A. Sarrafi, 1997. Evidence for genetic and media effect on haploid regeneration in the anther culture of hexaploid wheat (Triticum aestivum L.). Plant Breeding 116:502–504
Navarro-Alvarez, W., P.S. Baenziger, K.M. Eskridge, V.D. Gustafson and M. Hugo, 1994. Effect of sugars in wheat anther culture media. Plant Breeding 112:53–62
Ouyang, J., 1986. Induction of pollen plants in Triticum aestivum. In Haploids of higher plants in vitro. Science Press. Beijing., pp. 26–41
Roberts-Ochschlager, S.L., J.M. Dunwell and R. Foulks, 1990. Changes in the sugar content of barley anthers during culture on different carbohydrates. Plant Cell Tissue and Organ Culture. 22:77–83
Zhou, H, and C.F. Konzak, 1989. Improvement of anther culture methods for haploid production in wheat. Crop. Sci. 29:817–821
Zhou, H, Y. Zheng, Y and C.F. Konzak, 1991. Osmotic potential of media affecting green plant percentage in wheat anther culture. Plant Cell Rep. 10:63–66
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González, J.M., Jouve, N. Improvement of Anther Culture Media for Haploid Production in Triticale. CEREAL RESEARCH COMMUNICATIONS 28, 65–72 (2000). https://doi.org/10.1007/BF03543575
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DOI: https://doi.org/10.1007/BF03543575