Summary
Stripe rust (yellow rust) is one of the most devastating diseases of wheat worldwide. The most effective control method of stripe rust is the use of resistance genes. A very effective stripe rust resistance gene, Yr26, originating from Triticum turgidum L., was transferred into two common wheat (Triticum aestivum L.) cultivars of Turkey through backcrossing using closely linked microsatellite markers. A 6VS/6AL translocation line (92R149) was used as the source of Yr26 because this gene was originally thought to be located in the short arm of chromosome 6V. Wheat cultivars, Gerek-79 and Gün-91, were used as the recurrent parents in backcrossing. Resistant backcross lines were selected using closely linked chromosome IB microsatellite markers (Xgwm11 and Xgwm18). Resistant backcross lines were also screened with a 6VS specific SCAR marker (SCAR1265), and results reconfirmed that Yr26 was not carried on 6VS. Highly resistant BC4F3 plants of Gerek-79 and Gün-91 were produced and the existence of Yr26 was confirmed with the linked Xgwm11 and Xgwm18 microsatellite markers.
Similar content being viewed by others
References
Braun, H.J. & E.E. Saari, 1992. An assessment of the potential of Puccinia striiiformis f. sp. tritici to cause yield losses in wheat on the Anatolian Plateau of Turkey. Vortr Planzenzüchtg 24: 121–123.
Chen, P. D., L. L. Qi, B. Zhou, S. Z. Zhang & D. J. Liu, 1995. Development and molecular cytogenetic analysis of wheat-Haynaldia villosa 6VS/6AL translocation lines specifying resistance to powdery mildew. Theor Appl Genet 91: 1125–1128.
Dusunceli, F., L. Cetin, S. Albustan & S.P.S. Beniwal, 1996. Occurence and impact of wheat stripe rust (Puccinia striiformis) in Turkey in 1994–95 crop season. In: Proceedings of the 9th European and Mediterranean Cereal Rusts and Mildews Conference, 2–6 September 1996, Launteren, The Netherlands.
Incirli, A. & M.S. Akkaya, 2001. Assessment of Genetic Relationships in Durum Wheat Cultivars Using AFLP Markers. Gen Res Crop Evol 48: 233–238.
Liu, Z., Q. Sun, Z. Ni & T. Yang, 1999. Development of SCAR markers linked to the Pm21 gene conferring resistance to powdery mildew in common wheat. Plant Breeding 118: 215–219.
Ma, J., R. Zhou, Y. Dong, L. Wang, X. Wang & J. Jia, 2001. Molecular mapping and detection of the yellow rust resistance gene Yr26 in wheat transferred from Triticum turgidum L. using microsatellite markers. Euphytica 120: 219–226.
Mamluk, O.F. & M. El-Naimi, 1992. Occurence and virulence of wheat yellow rust in Syria. In: P.J. Zeller and O. Fishhook (Eds.), Proc 8th European and Mediterranean Cereal Rusts and Mildew Conference 8–11 September 1992, Wheihenstephan, Germany.
Mcintosh, R. A., G.E., Hart, K. M. Devos, M.D. Gale, and W. J. Rogers, 1998. Catalogue of gene symbols for wheat. In: A. E. Slinkard (Ed.), Proc 9th Int. Wheat Genet. Symp., Vol 5, University Extension Press, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Michelmore, R. W. 1995. Molecular approach to manipulation of disease resistance genes. Annu Rev Phytopathol 15: 393–427.
Peng, J.H., T. Fahima, M.S. Roder, Y.C. Li, A. Dahan, A. Grama, Y.I. Ronin, A.B. Korol & E. Nevo, 1999. Microsatellite tagging of the stripe rust resistance gene YrH52 derived from wild emmer wheat, Triticum dicoccoides, and suggestive negative crossover interference on chromosome 1B. Theor Appl Genet 98:862–872.
Röder, M.S., J. Plaschke, S.U. Konig, A. Borner, M.E. Sorrels, S.D. Tanksley & W.M. Ganal, 1995. Abundance, variability and chromosomal location of microsatellites in wheat. Mol Gen Genet 246: 327–333.
Röder, M.S., V. Korzun, K. Wendehake, J. Plaschke, M.H. Tixier, P. Leroy & W.M. Ganal, 1998. A microsatellite map of wheat. Genetics 149: 2007–2023.
Rong, J. K., E. Millet, J. Manisterski & M. Feldman, 2000. A new powdery mildew resistance gene: introgression from wild emmer into common wheat and RFLP-based mapping. Euphytica 115: 121–126.
Stubbs, R., W. Prescott., E.E. Saari & H.J. Dubin, 1986. Cereal Disease Methodology Manuel, IPO. Wageningen and CIMMYT, Mexico.
Torabi, M. & K. Nazari, 1998. Seedling and adult plant resistance to yellow rust in Iranian bread wheats. Euphytica 100: 51–54.
Wang, Z., J.L. Weber, G. Zhong & S.D. Tanksley, 1994. Survey of plant short tandem DNA repeats. Theor Appl Genet 88: 1–6.
Yildirim, A., S.S. Jones, T.D. Murray, T.S. Cox & R.F. Line, 1995. Resistance to stripe rust and eyespot diseases of wheat in Triticum tauschii. Plant Disease 79: 1230–1236.
Yildirim, A., S. Gokmen, HJ. Braun, H. Ketata & H. Ekiz, 1999. Importance of yellow rust disease of wheat in Turkey and breeding efforts. In: Orta Anadolu’da Hububat Tariminin Sorunlari ve Cozum Yolari Sempozyumu, 8–11 Haziran, Konya.
Yildirim, A., S.S. Jones, T.D. Murray & R.F. Line, 2000. Evaluation of Dasypyrum villosum populations for resistance to cereal eyespot and stripe rust pathogens. Plant Disease 84: 40–44.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Yildirim, A., Karadag, Y., Sakin, M.A. et al. Transfer of stripe rust resistance gene Yr26 to Turkish wheats using microsatellite markers. CEREAL RESEARCH COMMUNICATIONS 32, 25–30 (2004). https://doi.org/10.1007/BF03543276
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF03543276