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A short GC-rich sequence involved in deletion formation of cloned DNA in E. coli

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Abstract

A spontaneous deletion probably caused by rec A independent recombination between short-direct repeat sequences was observed in pUC19 plasmid carrying a piece of Streptomyces genomic DNA after culturing in liquid medium. The deletion removed an unknown portion of the cloned DNA and the BamHI-EcoRI part of the multiple cloning site with an additional flanking 111 bp from the vector. At the junction a 13 bp GC-rich DNA sequence highly homologous to a known spontaneous deletion hotspot was found.

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Authors and Affiliations

  1. Institute of Biology, University Medical School of Debrecen, Nagyerdei krt. 98, H-4012, Debrecen, Hungary

    Andrea Sümegi, Zsuzsanna Birkó, F. Szeszák, S. Vitális &  S. Birkó

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  1. Andrea Sümegi
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  2. Zsuzsanna Birkó
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  3. F. Szeszák
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  4. S. Vitális
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  5. S. Birkó
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Dedicated to the memory of Professor Gábor Szabó.

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Sümegi, A., Birkó, Z., Szeszák, F. et al. A short GC-rich sequence involved in deletion formation of cloned DNA in E. coli. BIOLOGIA FUTURA 48, 275–279 (1997). https://doi.org/10.1007/BF03543199

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  • DOI: https://doi.org/10.1007/BF03543199

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