Skip to main content
SpringerLink
Log in

Effects of veratridine and its derivatives on the Na-conducting channels in Helix neurons

  • Published:
Acta Biologica Hungarica Aims and scope Submit manuscript

Abstract

Effects of veratrum alkaloids were studied on the Na-channels of the land snail Helix pomatia. It was found that veratridine and its analogues depolarize the membrane due to the increased Na-permeability. The inactivation was shifted right along the voltage axis and the recovery from the inactivation was faster after veratridine treatment. After alkaloid treatment the selectivity of the Na-channel decreased, however, the selectivity sequence was not altered. The activation curve was not shifted. Veratridine derivatives, which appeared to be more effective on insects, had almost no effect on the Na-current.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Barnes, S., Hille, B. (1988) Veratridine modifies open sodium channels. J. Gen. Physiol. 91, 421–443.

    Article  CAS  Google Scholar 

  2. Catteral, W. A. (1980) Neurotoxins that act on voltage-sensitive sodium channels in excitable membranes. Ann. Rev. Pharm. Tox. 20, 15–43.

    Article  Google Scholar 

  3. Crosby, D. G. (1971) Minor insecticides of plant origin. In: Jacobson, M., Crosby, D. G. (eds) Naturally Occuring Insecticides. Dekker, New York, pp. 177–239.

    Google Scholar 

  4. Garber, S. S., Miller, C. (1987) Single Na+ channels activated by veratridine and batrachotoxin. J. Gen. Physiol. 89, 459–480.

    Article  CAS  Google Scholar 

  5. Hille, B. (1971) The permeability of the sodium channel to organic cations in myelinated nerve. J. Gen. Physiol. 58, 599–619.

    Article  CAS  Google Scholar 

  6. Huang, L.-Y. M., Moran, N., Ehrenstein, G. (1982) Batrachotoxin modifies the gating kinetics of sodium channels in internally perfused neuroblastoma cells. National Acad, of Sci. 79, 2082–2085.

    Article  CAS  Google Scholar 

  7. Huang, L.-Y. M., Moran, N., Ehrenstein, G. (1984) Gating kinetics batrachotoxin modified sodium channels in neuroblastoma cells determined from single-channel measurements. J. Biophys. 45, 313–322.

    Article  CAS  Google Scholar 

  8. Kostyuk, P. G., Krishtal, O. A., Shakhovalov, Yu. A. (1977) Separation of sodium and calcium currents in the somatic membrane of mollusc neurones. J. Physiol. 370, 545–568.

    Article  Google Scholar 

  9. Kruger, B. K., Worley, J. F., French, R. J. (1983) Single sodium channels from rat brain incorporated into planar lipid bilayer membranes. Nature 303, 172–175.

    Article  Google Scholar 

  10. Leibowitz, M. D., Sutro, J. B., Hille, B. (1986) Voltage-dependent gating of veratridine modified Na channels. J. Gen. Physiol. 87, 25–46.

    Article  CAS  Google Scholar 

  11. Leicht, R., Meves, H., Wellhöner, H.-H. (1971a) The effect of veratridine on Helix pomatia neurones. Pflügers Arch. 323, 50–62.

    Article  CAS  Google Scholar 

  12. Leicht, R., Meves, H., Wellhöner, H.-H. (1971b) Slow changes of membrane permeability in giant neurones of Helix pomatia. Pflügers Arch. 323, 63–79.

    Article  CAS  Google Scholar 

  13. Moczydlowski, E., Garber, S. S., Miller, C. (1984) Batrachotoxin activated Na+ channels in planar lipid bilayers. Competition of tetrodotoxin block by Na+. J. Gen Physiol. 84, 665–686.

    Article  CAS  Google Scholar 

  14. Naumov, A. P., Negulyaev, Yu. A., Nosyreva, E. D. (1979) Change of selectivity of sodium channels in membrane of nerve fiber treated with veratridine. Tsitologia 21, 692–696 (In Russian).

    CAS  Google Scholar 

  15. Ohta, M., Narahashi, T., Keeler, R. F. (1973) Effects of veratrum alkaloids on membrane potential and conductance of squid and crayfish giant axons. J. Pharmacol. Exp. Ther. 184, 143–154.

    CAS  Google Scholar 

  16. Quandt, F. N., Narahashi, T. (1982) Modification of single Na+ channels by batrachotoxin. National Acad, of Sci. 79, 6732–6736.

    Article  CAS  Google Scholar 

  17. Rando, T. A., Wang, G. K., Strichartz, G. R. (1986) The interaction between the activator agents batrachotoxin and veratridine and the gating processes of neuronal sodium channels. Mol. Pharmacol. 29, 467–477.

    PubMed  CAS  Google Scholar 

  18. Rando, T. A. (1989) Rapid and slow gating of veratridine-modified sodium channels in frog myelinated nerve. J. Gen. Physiol. 93, 43–65.

    Article  CAS  Google Scholar 

  19. Strichartz, G., Rando, T., Wang, G. K. (1987) An integrated view of the molecular toxicology of sodium channel gating in excitable cells. Ann. Rev. Neurosci. 10, 237–267.

    Article  CAS  Google Scholar 

  20. Sutro, J. B. (1986) Kinetics of veratridine action on Na channels of skeletal muscle. J. Gen. Physiol. 87, 1–24.

    Article  CAS  Google Scholar 

  21. Ujváry, I., Eya, B. K., Grendell, R. L., Toia, R. F., Casida, J. E. (1991) Insecticidal activity of various 3-acyl and other derivates of veracevine relative to the Veratrum alkaloids veratridine and cevadine. J. Agric. Food Chem. 39, 1875–1881.

    Article  Google Scholar 

  22. Ulbricht, W. (1969) The effect of veratridine on excitable membranes in nerve and muscle. Ergebnisse der Physiologie. 61, 17–71.

    Google Scholar 

  23. Ulbricht, W. (1972) Rate of veratridine action on the nodal membrane. I. Fast hase determined during sustained depolarization in the voltage clamp. Pflügers Arch. 336, 187–199.

    Article  CAS  Google Scholar 

  24. Ulbricht, W. (1972) Rate of veratridine action on the nodal membrane. II. Fast and slow phase determined with periodic impulses in the voltage clamp. Pflügers Arch. 336, 201–212.

    Article  CAS  Google Scholar 

  25. Yoshii, M., Narahashi, T. (1984) Patch clamp analysis of veratridine induced sodium channels. Biophys. J. 45, 184a (Abstr.).

    Google Scholar 

  26. Zong, X.-G., Dugas, M., Honerjager, P. (1992) The relation between veratridine reaction dynamics and macroscopic Na current in single cardiac cells. J. Gen. Physiol. 99, 683–697.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Balaton Limnological Research Institute of the Hungarian Academy of Sciences, H-8237, Tihany, P.O. Box 35, Hungary

    T. Kiss

  2. Plant Protection Institute of the Hungarian Academy of Sciences, Budapest, Hungary

    I. Ujváry

Authors
  1. T. Kiss
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. Ujváry
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Dedicated to Professor János Salánki for his 70th birthday.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kiss, T., Ujváry, I. Effects of veratridine and its derivatives on the Na-conducting channels in Helix neurons. BIOLOGIA FUTURA 50, 131–143 (1999). https://doi.org/10.1007/BF03543037

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03543037

Keywords

Search

Navigation