Summary
The Rhodobacter sphaeroides polyhydroxyalkanoate synthase structural gene (phaCRs) was identified as a 1,806-bp open reading frame. It encoded a PHA synthase of 601 amino acids with a relative molecular mass of 66,742, which exhibited 25.9 to 39.0 % amino acid identity with PHA synthases from other bacteria. Enzymatic analysis, nucleotide sequence analysis, hybridization studies and analysis of Tn5-induced PHA-negative mutants provided multiple evidence that phaCRs represents a monocistronic operon and that phaCRs is isolated in the genome from other genes known to be relevant for PHA metabolism. It is not clustered with the structural genes for β-ketothiolase or pyridine nucleotide dependent acetoacetyl-CoA reductase.
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Hustede, E., Steinbüchel, A. Characterization of the Polyhydroxyalkanoate Synthase Gene Locus of Rhodobacter Sphaeroides. Biotechnol Lett 15, 709–714 (1993). https://doi.org/10.1007/BF03500683
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DOI: https://doi.org/10.1007/BF03500683