Distribution of the Cereal Cyst Nematodes (Heterodera spp.) in Wheat and Barley Fields in North-Eastern Regions of Syria

Abstract

Cereal cyst nematodes (CCN) are important plant-parasitic nematodes of wheat and barley, and exist in most of the cereal growing regions of the world. As there is limited information on the nature and distribution of CCN species in Syria, a survey was conducted in north-eastern Syria to assess the distribution of CCN in the main wheat and barley growing areas. In the summer of 2009, a total of 167 composite soil samples were collected from 167 wheat and barley fields. Cysts were extracted from soil using the Fenwick can technique, then quantified and identified up to the species level by using both morphological and molecular methods. The study revealed that 62% of the fields were infested with the three Heterodera species: H. avenae, H. filipjevi and H. latipons. However, the most prevalent species was H. latipons, which was present in 76% of the infested samples, while it occurred alone in 67% of those samples. Heterodera avenae was detected singly in 20% of the samples, while 11% consisted of mixed populations with other species. Heterodera filipjevi was never detected alone; it was found mixed with H. avenae and/or H. latipons in 9% of the samples. The high number of extracted cysts (up to 116 cysts × 200 g−1 of soil) is most likely related to the monoculture practiced in that region and the fact that growers are unaware of the existence of resistant varieties.

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References

  1. Abidou H, El-Ahmed A, Nicol JM, Bolat N, Rivoal R & Yahyaoui A, 2005. Occurrence and distribution of species of the Heterodera avenae group in Syria and Turkey. Nematol Medit 33, 195–201.

    Google Scholar 

  2. Anonymous, 2010. Annual Agricultural Statistical Report. Directorate of Planning and Statistics, Ministry of Agriculture and Agrarian Reform. Department of Agricultural Economics. Syrian Central Office of Statistics.

    Google Scholar 

  3. Bekal S, Jahier J & Rivoal R, 1998. Host responses of different Triticeae to species of the cereal cyst nematode complex in relation to breeding resistant durum wheat. Fundam Appl Nematol 21, 359–370.

    Google Scholar 

  4. Brown RH, 1985. The selection of management strategies for controlling nematodes in cereals. Agric Ecosyst Environ 12, 371–388.

    CAS  Article  Google Scholar 

  5. Dababat AA, Imren M, Erginbas-Orakci G, Ashrafi S, Yavuzaslanoglu E, Toktay H, Pariyar SR, Elekcioglu HI, Morgounov AI & Mekete T, 2014. The importance and management strategies of cereal cyst nematodes, Heterodera spp., in Turkey. Euphytica 202, 173–188.

    Article  Google Scholar 

  6. Dixon J, Braun JH & Crouch JH, 2009. Overview: Transitioning wheat research to serve the future needs of the developing countries. In: Dixon J, Braun JH, Kosina P, Crouch JH, (Eds.), Wheat Facts and Futures 2009. Mexico, D.F., CIMMYT.

    Google Scholar 

  7. Fenwick DW, 1940. Methods for the recovery and counting of cysts of Heterodera schachtii from soil. J Helminthol 18, 155–172.

    Article  Google Scholar 

  8. Ferris VR, Ferris JM & Faghihi J, 1993. Variation in spacer ribosomal DNA in some cyst-forming species of plant parasitic nematodes. Fundam Appl Nematol 16, 177–184.

    Google Scholar 

  9. Hall TA, 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows95/98/NT. Nucleic Acids Symposium Series 41, 95–98.

    CAS  Google Scholar 

  10. Handoo ZA, 2002. A key and compendium to species of the Heterodera avenae group (Nematode: Heteroderidae). J Nematol 34, 250–262.

    PubMed  PubMed Central  Google Scholar 

  11. Hassan GA, 2008. Ecological and biological study of cyst nematodes (Heterodera spp.) on wheat in Al-Hassakah Governorate. M.Sc. thesis. Damascus University, Syria.

    Google Scholar 

  12. Hassan GA, Al-Assas K & Jamal M, 2010. Damage potential and reproduction of Heterodera avenae on wheat under Syrian field conditions. Nematol Medit 38, 73–78.

    Google Scholar 

  13. Holterman M, van der Wurff A, van den Elsen S, van Megen H, Bongers T, Holovachov O, Bakker J & Helder J, 2006. Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown clades. Mol Biol Evol 23, 1792–1800.

    CAS  Article  PubMed  Google Scholar 

  14. Ibrahim AAM, Al Hazmi AS, Al Yahya FA & Alderfasi AA, 1999. Damage potential and reproduction of Heterodera avenae on wheat and barley under Saudi field conditions. Nematology 1, 625–630.

    Article  Google Scholar 

  15. Imren M, Waeyenberge L, Viaene N, Toktay H, Dababat A & Elekcioǧlu H, 2012. Molecular characterization of cereal cyst nematodes from South Anatolian Region in Turkey using ITS-rDNA sequences. Turk J Entomol 4, 491–499.

    Google Scholar 

  16. Mokabli A, Valette S, Gauthier JP & Rivoal R, 2002. Variation in virulence of cereal cyst nematode populations from North Africa and Asia. Nematology 4, 521–525.

    Article  Google Scholar 

  17. Nicol JM & Rivoal R, 2008. Global knowledge and its application for the integrated control and management of nematodes on wheat. In: Ciancio A and Mukerji KG (Eds). Integrated management and biocontrol of vegetable and grain crops nematodes, Vol. 2. Dordrecht, The Netherlands, Springer, 243–287.

    Google Scholar 

  18. Nicol JM, Rivoal R, Taylor S & Zaharieva M, 2004. Global importance of cyst (Heterodera spp.) and lesion nematode (Pratylenchus spp.) on cereals: distribution, yield loss, use of host resistance and integration of molecular tools. Nematology Monographs and Perspectives 2, 1–19.

    Google Scholar 

  19. Öztürk G, Yildirim AF & Enneli S, 1998. Distribution and frequency of cereal cyst nematodes (H. avenae Wollenweber) in Konya wheat growing area. Proceedings of Turkey Phytopatology Congress, Ankara, Turkey, 260–264.

    Google Scholar 

  20. Peng D, Nicol JM, Zhang D, Chen S, Waeyenberge L & Moens M, 2007. Occurrence, distribution and research situation of cereal cyst nematode in China. [Abstract]. Proc. Int. Plant Protection Conf., 16th, Glasgow, Scotland, UK. 15–18 Oct. 2007. Br. Crop Prod. Counc., Alton, Hampshire, UK. 350–351.

    Google Scholar 

  21. Rivoal R, Bekal S, Valette S, Gauthier JP, Bel Hadj Fradj M, Mokabli, A, Jahier J, Nicol JM & Yahyaoui A, 2001. Variation in reproductive capacity and virulence of different genotypes and resistance genes of Triticeae, in the cereal cyst nematode complex. Nematology 3, 581–592.

    Article  Google Scholar 

  22. Rivoal R, Valette S, Bekal S, Gauthier JB & Yahyaoui A, 2003. Genetic and phenotypic diversity in the graminaceous cyst nematode complex, inferred from PCR-RFLP of ribosomal DNA and morphometric analysis. Eur J Plant Pathol 109, 227–241.

    CAS  Article  Google Scholar 

  23. Sahin E, Nicol JM, Elekçioǧlu IH, Yorgancilar O, Yildirim AF, Tülek A, Hekimhan H, Yorgancilar A, Kilinç AT, Bolat N & Erginbaç-Orakci G, 2009. Frequency and diversity of cereal Nematodes on the central Anatolian Plateau of Turkey. Proceedings of the First Workshop of the International Cereal Cyst Nematode Initiative, 21–23 October 2009 (pp. 100–105). Antalya, Turkey, CIMMYT.

    Google Scholar 

  24. Schölz U, 2001. Biology, pathogenicity and control of the cereal cyst nematode Heterodera latipons Franklin on wheat and barley under semiarid conditions, and interactions with common root rot Bipolaris sorokiniana (Sacc.) Shoemaker [teleomorph: Cochliobolus sativus (Ito et Kurib.) Drechs. ex Dastur.]. Ph.D. thesis. Bonn University, Bonn, Germany.

    Google Scholar 

  25. Smiley RW, Ingham RE, Uddin W & Cook GH, 1994. Crop sequences for winter wheat in soil infested with cereal cyst nematode and fungal pathogens. Plant Dis 78, 1142–1149.

    Article  Google Scholar 

  26. Smiley RW & Nicol JM, 2009. Nematodes which challenge global wheat production. In Wheat Science and Trade. Carver, BF (Ed.). Wiley-Blackwell, Ames, IA. 171–187.

    Google Scholar 

  27. Smiley RW, Whittaker RG, Gourlie JA, Easley SA & Ingham RE, 2005. Plant-parasitic nematodes associated with reduced wheat yield in Oregon: Heterodera avenae. J Nematol 37, 297–307.

    PubMed  PubMed Central  Google Scholar 

  28. Taheri A, Hollamby GJ, Vanstone A & Neate SM, 1994. Interaction between root lesion nematode, Pratylenchus neglectus (Rensch 1924) Chitwood and Oteifa 1952, and root rotting fungi of wheat. NZ J Crop Hortic Sci 22, 181–185.

    Article  Google Scholar 

  29. Toumi F, Waeyenberge L, Viaene N, Dababat A, Nicol JM, Ogbonnaya F & Moens M, 2013a. Development of a species-specific PCR to detect the cereal cyst nematode Heterodera latipons. Nematology 15, 709–717.

    CAS  Google Scholar 

  30. Toumi F, Waeyenberge L, Viaene N, Dababat A, Nicol JM, Ogbonnaya F & Moens M, 2013b. Development of two species-specific primer sets to detect the cereal cyst nematodes Heterodera avenae and Heterodera filipjevi. Eur J Plant Pathol 136, 613–624.

    CAS  Article  Google Scholar 

  31. Yavuzaslanoglu E, Elekçioǧlu H, Nicol, JM, Yorgancilar O, Hodson D, Yildirim FA, Yorgancilar A & Bolat B, 2012. Distribution, frequency and occurrence of cereal nematodes on the Central Anatolian Plateau in Turkey and their relationship with soil physicochemical properties. Nematology, 14, 839–854.

    Article  Google Scholar 

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Correspondence to Fateh Toumi.

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Toumi, F., Hassan, G., Waeyenberge, L. et al. Distribution of the Cereal Cyst Nematodes (Heterodera spp.) in Wheat and Barley Fields in North-Eastern Regions of Syria. J Plant Dis Prot 122, 255–263 (2015). https://doi.org/10.1007/BF03356561

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Key words

  • cereals
  • Heterodera avenae
  • H. filipjevi. H. latipons
  • survey