Abstract
Citrus tristeza virus (CTV) infection in 13 Satsuma (Citrus unshiu (Mak.) Marc.) symptomless clones was investigated by different diagnostic methods, namely ELISA (Enzyme Linked Immunosorbent Assay), DTBIA (Direct Tissue Blot Immunoassay), and biological testing. The objective was to correlate the outcomes of the two serological tests mutually and with the severity of tristeza disease symptoms on indicator plants. ELISA and DTBIA were performed on the clones and on inoculated tissue samples of Mexican lime, while biological testing was performed on four citrus indicators. An additional goal of this study was to evaluate the efficiency of CTV DTBIA testing using different tissue types, including Satsuma immature ovaries and flower petioles. ELISA performed on spring shoots tissue of Mexican lime and DTBIA performed on mature ovaries of Satsuma field samples, showed high intensity of reactions and detection efficiency. Both tests were equally reliable and would make a good choice for fast CTV detection.
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Hancevic, K., Cerni, S., Radic, T. et al. Comparison of different methods for Citrus tristeza virus detection in Satsuma mandarins. J Plant Dis Prot 119, 2–7 (2012). https://doi.org/10.1007/BF03356412
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DOI: https://doi.org/10.1007/BF03356412