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Multiplication and movement of Xanthomonas axonopodis pv. vignicola and seed contamination in cowpea (Vigna unguiculata) genotypes

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Abstract

The multiplication and systemic distribution of two Xan-thomonas axonopodis pv. vignicola strains from Benin differing in virulence were monitored after inoculation via leaf infiltration until 25 days post inoculation (dpi) in the susceptible cowpea genotype IT84E-124. The virulent strain multiplied fast in leaves to yield a final population density of about 109 colony forming units (cfu) cm−2 of leaf, spread through the whole aerial part of the plant within two weeks (106 cfu g−1 of stem) and caused blight, also on non-inoculated leaves at 15 dpi. In contrast, the less virulent strain multiplied slowly in leaves with a final population of about 102 cfu cm−2 of leaf and did not spread through the whole plant until 25 dpi, colonising only the lower stem portion with 2.4 × 104 cfu g−1 of stem.

Comparing cowpea genotypes IT84E-124 (susceptible), IT86D-715 (moderately resistant) and IT86D-719 (resistant), considerable differences in bacterial numbers were obtained after infection. At 15 dpi, bacteria had spread in high densities through the whole plant in genotype IT84E-124, in leaves and occasionally in stems in genotype IT86D-715, but in genotype IT86D-719 bacteria were limited to the inoculated leaves. In genotypes IT86D-715 and IT86D-719, bacteria moved predominantly upwards through stems from the point of onset of the inoculated leaf.

Contamination of seeds derived from infected plants, varied with the resistance level of cowpea genotypes. There was no relationship between contamination and disease severity. Lower bacteria densities were found in seeds from plants with low disease severity, and no bacteria were detected in seeds of genotypes with high or low disease severity. Most bacteria were concentrated on the seed surface, and only few were found inside the seed under the seed coat. Typical ‘water soaked spots’ appeared on 17% of seedlings emerging from contaminated seeds of genotype IT86D-472. In conclusion, movement of bacteria on or into seeds depended on genotypes, and seed contamination did not always result in seedling infection. In breeding for resistance, the contamination of seeds and infection of seeds in symptomless plants in apparently resistant genotypes should be determined and considered.

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References

  • Allington WB & Chamberlain DW, 1949. Trends in the populations of pathogenic bacteria within leaf tissue of susceptible and immune plant species. Phytopathology 39, 656–660.

    Google Scholar 

  • Bochner BR, 1989. Product review: Sleuthing out bacterial identities. Nature 339, 157–158.

    Article  CAS  PubMed  Google Scholar 

  • Cafati, CR & Saettler AW, 1980a. Role of nonhost species as alternate inoculum sources of Xanthomona sphaseoli. Plant Dis 64, 194–196.

    Article  Google Scholar 

  • Cafati, CR & Saettler AW, 1980b. Effect of host on multiplication and distribution of bean common blight bacteria. Phytopathology 70, 675–679.

    Article  Google Scholar 

  • Daniel JF, 1991. Contribution à l’Etude de la Biologie de Xanthomonas campestris pathovar manihotis (ARTHAUD-BERTHET et BONDAR) STARR Agent Responsable de la BacterioseVasculaire du Manioc, Manihot esculenta CRANTZ. Dissertation, Université de Paris-Sud Centre d’Orsay.

    Google Scholar 

  • Darrasse A, Bureau C, Samson R, Morris CE & Jacques MA, 2007. Contamination of bean seeds by Xanthomonas axo-nopodis pv. phaseoli associated with low bacterial densities in the phyllosphere under field and greenhouse conditions. Eur J Plant Pathol 119, 203–215.

    Article  Google Scholar 

  • Darrasse A, Darsonval A, Durand K, Boureau T, Jacques M-A & Brisset M-N, 2010. Transmission of Plant-Pathogenic Bacteria by Nonhost Seeds without Induction of an Associated Defense Reaction at Emergence. Appl Environ Microbiol 76, 6787–6796.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Darsonval A, Darrasse A, Meyer D, Demarty M, Durand K, Bureau C, Manceau C & Jacques M-A, 2008. Type III secretion system of Xanthomonas fuscans subsp. fuscans is involved in phyllosphere colonization process and in transmission to seeds of susceptible bean. Appl Environ Microbiol 74, 2669–2678.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gitatis RD, 1982. Resistance in cowpea to bacterial blight and canker. Phytopathology 72, 999.

    Google Scholar 

  • Hokawat S & Rudolph K, 1991. Variation in pathogenicity and virulence of strains of Xanthomonas campestris pv. glycines, the incitant of bacteria pustule of soybean. J Phytopathol 131, 73–83.

    Article  Google Scholar 

  • Hopkins DL, Thompson CM, Hilgren J & Lovic B, 2003. Wet seed treatment with peroxyacetic acid for the control of bacterial fruit blotch and other seedborne diseases of watermelon. Plant Dis 87, 1495–1499.

    Article  CAS  Google Scholar 

  • Jindal JK, Patel PN & Khan AM, 1981. Variability in xantho-monads of grain legumes. Phythopath Z 100, 1–9.

    Article  Google Scholar 

  • Jindal JK & Patel PN, 1984. Variability in xanthomonads of grain legumes. IV. Variations in bacteriological properties of 83 isolates and pathogenic behavior of cultural variants. Phytopath Z 110, 63–68.

    Article  Google Scholar 

  • Kaiser W J & Ramos AH, 1979. Two bacterial diseases of cowpea in East Africa. Plant Dis Reptr 63, 304–308.

    Google Scholar 

  • Khatri-Chhetri G, Wydra K & Rudolph K, 1998. Variability of strains of Xanthomonas campestris pv. vignicola, incitant of cowpea bacterial blight and bacterial pustule, collected in several African and other countries. In: Mahadevan, A (Ed.) 1998: Plant Pathogenic Bacteria. Centre Adv. Study Botany, Univ. Madras, India. 296–302.

    Google Scholar 

  • Khatri-Chhetri G, 1999. Detection and characterization of Xanthomonas campestris pv. vignicola strains, incitant of Cowpea Bacterial Blight and Pustule, and studies on Genotype/Strain-interactions. PhD thesis-University of Göttingen, Germany.

    Google Scholar 

  • Kishun R, 1989. Appraisal of loss in yield of cowpea due to Xanthomonas campestris pv. vignicola. Indian Phytopathology 42, 241–246.

    Google Scholar 

  • Kotchoni OS, Torimiro N & Gachomo EW, 2007. Control of Xanthomonas campestris pv. vignicola in cowpea following seed and seedling treatment with hydrogen peroxide and N-Heterocyclic pyridinium chlorochromate. J Plant Pathol 89, 361–367.

    CAS  Google Scholar 

  • Okechukwu RU, Ekpo EJA & Okechukwu OC, 2010. Seed to plant transmission of Xanthomonas campestris pv. vignicola isolates in cowpea. Afr J Agric Res 5, 431–435.

    Google Scholar 

  • Omer MEH & Wood RKS, 1969. Growth of Pseudomonas phaseolicola in susceptible and resistant bean plants. Ann Appl Biol 63, 103–116.

    Article  Google Scholar 

  • Patel PN, 1981. Pathogen variability and host resistance in bacterial pustule disease of cowpea in Africa. Trop Agric 58, 275–280.

    Google Scholar 

  • Patel PN, 1982. Genetics of host reactions to three races of the bacterial pustule pathogen in cowpea. Euphytica 31, 805–814.

    Article  Google Scholar 

  • Rudolph K, 1980. Multiplication of bacteria in leaf tissue. Angew Botanik 54, 1–9.

    Google Scholar 

  • Saettler AW, 1989. Common bacterial blight. In: Schwartz, HF, Pasto-Corrales MA (Eds.) 1989: Bean Production Problems in the Tropics, 2nd edn. Centro Internacional de Agricultura Tropical, Cali, Colombia. 261–283.

    Google Scholar 

  • Shekhawat GS & Patel PN, 1977. Seed transmission and spread of bacterial blight of cowpea and bacterial leaf spot of green gram in summer and monsoon seasons. Plant Dis Reptr 61, 390–392.

    Google Scholar 

  • Sikirou R, 1999. Epidemiological investigations and development of integrated control methods of bacterial blight of cowpea caused by Xanthomonas campestris pv. vignicola. PhD thesis-University of Göttingen, Germany.

    Google Scholar 

  • Sikirou R, Wydra K & Rudolph K, 2001. Selection of cowpea genotypes resistant to bacterial blight caused by Xanthomonas campestris pv. vignicola. In: Deboer, S (Ed.) 2001: Plant Pathogenic Bacteria. Kluwer Academic Publishers, Dordrecht, Netherlands. 309–314.

    Chapter  Google Scholar 

  • Stadt SJ & Saettler AW, 1981. Effect of host genotype on multiplication of Pseudomonas phaseolicola. Phytopathology 71, 1307–1310.

    Google Scholar 

  • Thind BS, Soni PS & Kumar K, 1984. Seed transmission of Xanthomonas campestris pv. vignaeradiatae in mungbean and X. campestris pv. vignicola in cowpea. Indian Phytopathol 37, 414 (abstr.).

    Google Scholar 

  • Vakili NG, Kaiser WJ, Perez JA & Cortes-Monllor A, 1975. Bacterial blight of beans caused by two Xanthomonas pathogenic types from puerto Rico. Phytopathology 65, 401–403.

    Google Scholar 

  • Vauterin L, Hoste B, Kersters K & Swings J, 1995. Reclassification of Xanthomonas. Int J System Bacteriol 45, 472–489.

    Article  CAS  Google Scholar 

  • Wydra K, 1991. Interaktionen zwischen Zellwandpolymeren von Bohnenpflanzen und Zellen von Pseudomonas syringae pv. Phaseolicola sowie deren extrazellulären, polymeren Produkten. PhD thesis. Institute of Plant Pathology and Plant Protection, Faculty of Agricultural Sciences, University of Göttingen.

    Google Scholar 

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Author notes

  1. Rachidatou Sikirou

    Present address: Laboratoire de Défense des Cultures, Centre de Recherches Agricoles d’Agonkanmey, Institut National des Recherches Agricoles du Bénin (INRAB), 01 BP 128, Porto-Novo, Republic of Benin

  2. Kerstin Wydra

    Present address: Centre for Tropical and Subtropical Agriculture and Forestry (CeTSAF) — Tropenzentrum, Georg-August Universität Göttingen, Büsgenweg 1, D-37077, Göttingen, Germany

Authors and Affiliations

  1. Plant Health Management Division, International Institute of Tropical Agriculture (IITA), 08 Tri Postal, 0932, Cotonou, Republic of Benin

    Rachidatou Sikirou & Kerstin Wydra

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  1. Rachidatou Sikirou
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Correspondence to Rachidatou Sikirou.

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Sikirou, R., Wydra, K. Multiplication and movement of Xanthomonas axonopodis pv. vignicola and seed contamination in cowpea (Vigna unguiculata) genotypes. J Plant Dis Prot 118, 93–101 (2011). https://doi.org/10.1007/BF03356388

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  • DOI: https://doi.org/10.1007/BF03356388

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