Abstract
Bean common mosaic virus (BCMV) is a major seed transmitted virus of common bean (Phaseolus vulgaris). The use of virus-free germplasm is a prerequisite for production of certified seeds, which is an important disease control measure. In this study, the efficiency of electrotherapy in eliminating BCMV and producing virus-free plants in two common bean cultivars, Khomein and Capsouli, was evaluated. Nodal cuttings of infected bean plants were exposed to electric currents of 5, 10 and 15 mA for 10 minutes and planted in vitro. One month old regenerated plantlets were tested by a Double Antibody Sandwich (DAS)-ELISA method for BCMV infection. The three intensities of electric current resulted in mean virus elimination percentages of 37.2%, 45.5% and 71.9% and mean plant regeneration of 79.5%, 68.6% and 58.4%, respectively. The Therapy Efficiency Index for the three electrotherapy treatments was estimated as 28.9, 29.2 and 39.1 in cv Khomein and 26.9, 33.3 and 44.8 in cv Capsouli, respectively. Based on the Therapy Efficiency Indices, an electric current of 15 mA for 10 min was the most effective treatment in both cultivars. The technique developed in this study can be a useful procedure for the production of virus-free germplasm and/or nucleus seed stock in crop plants infected with seed-borne viruses.
Zusammenfassung
Das ewöhnliche Bohnenmosaik-Virus (BCMV) ist eine bedeutende samenbürtige Viruskrankheit bei Phaseolus vulgaris. Die Benutzung von virusfreiem Pflanzmaterial ist Voraussetzung für die Produktion von zertifiziertem Saatgut und ist damit eine wichtige Kontrollmöglichkeit gegenüber dieser Krankheit. In dieser Arbeit wird die Effizienz der Elektrotherapie zur Bekämpfung der BCMV geprüft und virusfreie Phaseolus vulgaris-Pflanzen der Sorten Khomein und Capsouli erzeugt. Stängelabschnitte von infizierten Bohnenpflanzen wurden für 10 min mit Stromstärken von 5, 10, oder 15 mA behandelt und anschliend in in vitro-Kultur übertragen. Die regenerierten, einen Monat alten Jungpflanzen wurden mit einem Doppelantikörpersandwich-(DAS)-ELISA-Test hinsichtlich der BCMV-Infektion getestet. Bei den drei untersuchten Stromstärken konnte eine Unterdrückung der Viren von 37.2%, 45.5% und 71.9% erzielt werden, die durchschnittliche Pflanzenregeneration betrug 79.5%, 68.6% und 58.4%. Mit der Behandlung wurden Effizienzindices von 28.9, 29.2 und 39.1 bei der Sorte Khomein ermittelt sowie 26.9, 33.3 und 44.8 bei der Sorte Capsouli. Hinsichtlich der erzielten Effizienzindices ist die 10 minütige Behandlung mit einer Stromstärke von 15 mA die effektivste Anwendung für beide untersuchten Sorten. Für Nutzpflanzen, die mit samenbürtigen Viren belastet sind, kann die hier entwickelte Technik eine wertvolle Maßnahme für die Herstellung von virusfreiem Pflanzenmaterial sein.
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Hormozi-Nejad, M.H., Mozafari, J. & Rakhshandehroo, F. Elimination of Bean common mosaic virus using an electrotherapy technique. J Plant Dis Prot 117, 201–205 (2010). https://doi.org/10.1007/BF03356361
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DOI: https://doi.org/10.1007/BF03356361