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Control of the Colorado potato beetle (Leptinotarsa decemlineata [Say]) on potato under field conditions: a comparison of the efficacy of foliar application of two strains of Steinernema feltiae (Filipjev) and spraying with thiametoxam

Bekämpfung des Kartoffelkäfers (Leptinotarsa decemlineata [Say]) an Kartoffeln im Feld: ein Vergleich der Wirksamkeit von Blattbehandlungen mit zwei Stämmen von Steinernema feltiae (Filipjev) mit Thiametoxam

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The efficacy of entomopathogenic nematodes in controlling Leptinotarsa decemlineata on potato was tested in a field experiment. A Slovenian strain of Steinernema feltiae (B30) was used with the commercial product Entonem and the insecticide thiametoxam. The application of the nematodes and thiametoxam was repeated twice. In the second application half of the first dose (250.000 infective juveniles m–2) was used. Observing the population dynamics of the pest, it has become apparent that entomopathogenic nematodes significantly decreased the number of larvae, while no effect on their eggs and adults was confirmed. In controlling larvae in both years thiametoxam showed the best results in general, meanwhile treatments with EPNs showed no differences. In both years the highest yield was obtained when thiametoxam was used (2008: 24.38 t ha–1; 2009: 21.60 t ha–1), while the lowest yield was in the control treatment (2008: 7.65 t ha–1; 2009: 6.94 t ha–1). Different treatments of EPNs showed differences, and yields ranged from 16.44 (S. feltiae B30 low concentration) to 17.81 t ha–1 (Entonem high concentration) in 2008, and between 11.06 (S. feltiae B30 low concentration) and 14.88 t ha–1 (Entonem high concentration) in 2009. Both strains of entomopathogenic nematodes showed sufficient activity when controlling larvae, but unfortunately the high price of these biological agents at the moment makes it difficult for their broader implementation in the systems of potato production.


Die Wirksamkeit entomopathogener Nematoden bezüglich der Bekämpfung des Kartoffelkäfers (Leptinotarsa decemlineata) wurde im Feld an Kartoffeln untersucht. Ein slowenischer Stamm von Steinernema feltiae (B30) und das kommerzielle Produkt Entonem wurden mit dem Insektizid Thiametoxam in zweifacher Wiederholung verglichen. Bei der zweiten Anwendung wurde mit 250.000 Nematodenlarven m–2 die halbe Dosis der ersten ausgebracht. Populationsdynamische Beobachtungen am Schadtier ergaben, dass die entomopathogenen Nematoden zwar die Anzahl der Käferlarven signifikant verminderten, die Anzahl der Eier und Adulten aber nicht beeinflussten. Gegenüber den Käferlarven zeigte Thiametoxam in beiden Jahren generell die stärkste Wirkung, während sich die Wirkung der Varianten mit entomopathogenen Nematoden nicht voneinander unterschied. Die höchsten Kartoffelerträge wurden in beiden Versuchsjahren mit Thiametoxam erzielt (2008: 24,38 t ha–1; 2009: 21,60 t ha–1), während die geringsten Mengen in der Kontrolle geerntet wurden (2008: 7,65 t ha–1; 2009: 6,94 t ha–1). Die verschiedenen Behandlungen mit Nematoden wirkten unterschiedlich; die Erträge variierten zwischen 16,44 (S. feltiae B30 in geringer Dosierung) und 17,81 t ha–1 (Entonem in hoher Dosierung) im Jahr 2008, sowie zwischen 11,06 (S. feltiae B30 in geringer Dosierung) und 14,88 t ha–1 (Entonem in hoher Dosierung) im Jahr 2009. Beide untersuchten Nematodenstämme zeigten gegenüber den Larven des Kartoffelkäfers ausreichende Wirksamkeit, aber ihr derzeitig hoher Preis beeinträchtigt noch ihre verbreitetere Anwendung im Kartoffelbau.

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  • Ábrahám, E.B., E. Zsom, M. Sárvári, 2006: The effect of year and irrigation on the yield quantity and quality of the potato. J. Agric. Sci., Debrecen, 24, 12–16.

    Google Scholar 

  • Armer, C.A., R.E. Berry, G.L. Reed, S.J. Jepsen, 2004: Colorado potato beetle control by application of the entomopathogenic nematode Heterorhabditis marelata and potato plant alkaloid manipulation. Entomol. Exp. Appl. 111, 47–58.

    Article  Google Scholar 

  • Arthurs, S., K.M. Heinz, J.R. Prasifka, 2004: An analysis of using entomopathogenic nematodes against above-ground pests. Bull. Entomol. Res. 94, 297–306.

    CAS  PubMed  Google Scholar 

  • Bednarek, A., T. Nowicki, 1986: Effect of intrapopulation factors in the nematodes Steinernema feltiae (Steinernematidae) on the intensity of insect infestation. Zesz. Probl. Postepow Nauk Roln. 323, 199–212.

    Google Scholar 

  • Berry, R.E., 1998: Insects and Mites of Economic Importance in the Nortwest, 2nd edn. Oregon State University, Corvallis, OR, USA.

    Google Scholar 

  • Berry, R.E., J. Liu, G. Reed, 1997: Comparison of endemic and exotic entomopathogenic nematode species for control of Colorado potato beetle (Coleoptera: Chrysomelidae). J. Econ. Entomol. 90, 1528–1533.

    Article  CAS  PubMed  Google Scholar 

  • Cantelo, W.W., W.R. Nickle, 1992: Susceptibility of prepupae of the Colorado potato beetle (Coleoptera: Chrysomelidae) to entomopathogenic nematodes (Rhabditida: Steinernematidae, Heterorhabditidae). J. Entomol. Sci. 27, 37–43.

    Google Scholar 

  • Chavarría-Hernandez, N., M. De La Torre, 2001: Population growth kinetics of the nematode, Steinernema feltiae, in submerged monoxenic culture. Biotechnol. Lett. 23, 311–315.

    Article  Google Scholar 

  • Cranshaw, W.S., E.B. Radcliffe, 1980: Effect of defoliation on yield of potatoes. J. Econ. Entomol. 73, 131–134.

    Article  Google Scholar 

  • Dent, D., 2002: Can biological control replace chemicals? Pestic. News 58, 17.

    Google Scholar 

  • Ehlers, R.-U., 1998: Entomopathogenic nematodes–Save biocontrol agents for sustainable systems. Phytoprotection 79, 94–102.

    Article  Google Scholar 

  • Glazer, I., M. Klein, A. Navon, Y. Nakache, 1992: Comparison of efficacy of entomopatohgenic nematodes combined with antidesiccants applied by canopy sprays against three cotton pests (Lepidoptera, Noctuidae). J. Econ. Entomol. 85, 1636–1641.

    Article  Google Scholar 

  • Hare, J.D., 1980: Impact of defoliation by the Colorado potato beetle on potato yields. J. Econ. Entomol. 73, 369–373.

    Article  Google Scholar 

  • Hare, J.D., 1990: Ecology and management of the Colorado potato beetle. Annu. Rev. Entomol. 35, 81–100.

    Article  Google Scholar 

  • Hoffmann, E.J., S.M. Middleton, J.C. Wise, 2008: Ovicidal activity of organophosphate, oxadiazine, neonicotinoid and insect growth regulator chemistries on northern strain plum curculio, Conotrachelus nenuphar. J. Insect Sci. 8, 1536–2442.

    Article  Google Scholar 

  • Hough-Goldstein, J.A., G.E. Heimpel, H.E. Bechmann, C.E. Mason, 1993: Arthropod natural enemies of the Colorado potato beetle. Crop Prot. 12, 324–334.

    Article  Google Scholar 

  • Igrc-Barčič, J., R. Dobrinčč, M. Maceljski, 1999: Effect of insecticides on the Colorado potato beetles resistant to OP, OC and P insecticides. Anz. Schädlingskd. 72, 76–80.

    Google Scholar 

  • Ishibashi, N., D.-R. Choi, 1991: Biological control of soil pests by mixed application of entomopathogenic and fungivorous nematodes. J. Nematol. 23, 175–181.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Journey, A.M., K.R. Ostlie, 2000: Biological Control of Western Corn Rootworm (Coleoptera: Crysomelidae) Using the Entomopathogenic Nematode, Steinernema carpocapsae. Biol. Control 29, 822–831.

    Google Scholar 

  • Kakaty, B.M., S. Thamburaj, P. Stalin, 1992: Study on the growth and yield of sweet potato at different stages of harvest. J. Root Crops 18, 73–76.

    Google Scholar 

  • Kaya, H.K., 1990: Soil ecology. In: R. Gaugler, H.K. Kaya (eds.): Entomopathogenic Nematodes in Biological Control, pp. 93–115. CRC Press, Boca Raton, FL, USA.

    Google Scholar 

  • Kos, K., B. Tschöpe, E. Jörg, S. Trdan, 2009: Testing the suitability of SIMLEP decision support system for protection of potato against Colorado potato beetle (Leptinotarsa decemlineata [Say], Coleoptera, Chrysomelidae) in Slovenia. Acta Agric. Slov. 93, 93–104 [In Slovenian].

    Google Scholar 

  • Laznik, Ž., T. Tóth, T. Lakatos, M. Vidrih, S. Trdan, 2009: First record of Steinernema feltiae (Filipjev) (Rhabditida: Steinernematidae) in Slovenia. Helminthologia 46, 135–138.

    Article  Google Scholar 

  • Laznik, Ž., T. Tóth, T. Lakatos, M. Vidrih, S. Trdan, 2010: The activity of three new strains of Steinernema feltiae against adults of Sitophilus oryzae under laboratory conditions. J. Food Agric. Environ. 8, 132–136.

    Google Scholar 

  • Lebeck, L.M., R. Gaugler, H.K. Kaya, A.H. Hara, M.W. Johnson, 1993: Host stage suitability of the leafminer Liriomyza trifolii (Diptera: Agromyzidae) to the entomopathogenic nematode Steinernema carpocapsae (Rhabditida: Steinernematidae). J. Invertebr. Pathol. 62, 58–63.

    Article  Google Scholar 

  • Lopez, R., D.N. Ferro, R.G. Van Driesche, 1995: Two tachinid species discriminate between parasitized and non-parasitized hosts. Entomol. Exp. Appl. 74, 37–45.

    Article  Google Scholar 

  • MacVean, C.M., J.W. Brewer, J.L. Capinera, 1982: Field test of antidesiccants to extend the infection period of an entomogenous nematode, Neoaplectana carpocapsae, against the Colorado potato beetle. J. Econ. Entomol. 75, 97–101.

    Article  Google Scholar 

  • Mannan, M.A., M.K.R. Bhuiyan, A. Quasem, M.M. Rashid, M.A. Siddique, 1992: Study on the growth and partitioning of dry matter in sween potato. J. Root Crops 18, 1–5.

    Google Scholar 

  • Miloševič, D., I. Djalovič, Z. Bugarčič, 2008: The importance of a healthy planting material for potato production increases. Agro-Knowl. J. 9, 5–17.

    Google Scholar 

  • MINISTRY of AGRICULTURE, FOOD, and FORESTRY of REPUBLIC SLOVENIA [Maff], 2008: Decision on the change of the status of the exotic agent for biological control (no. 3430-9/ 2008/5) [In Slovenian].

  • Musa, F., D. Bericha, B. Kelmendi, I. Rusinovci, O. Zhitia, R. Bekqeli, I. Lushi, 2009: Tuber yield and other relevant parameters of some Netherlands potato varieties in agro-climatic conditions of Kosova. Bull. 44th Croatian and 4th Int. Symp. Agric., 444–448.

  • Pap, L., A. Toth, S. Karikas, 1997: A survey of the insecticide resistance status of the Colorado potato beetle, Leptinotarsa decemlineata, in Hungary between 1987 and 1991: Pestic. Sci. 49, 389–392.

    CAS  Google Scholar 

  • Stewart, J.G., G. Boiteau, J. Kimpinski, 1998: Management of late-season adults of the Colorado potato beetle (Coleoptera: Chrysomelidae) with entomopathogenic nematodes. Can. Entomol. 130, 509–514.

    Article  Google Scholar 

  • Trdan, S., M. Vidrih, N. Valič, 2006: Activity of four entomopathogenic nematode species against young adults of Sitophilus granarius (Coleoptera: Curculionidae) and Oryzaephilus surinamensis (Coleoptera: Silvanidae) under laboratory conditions. J. Plant Dis. Protect. 113, 168–173.

    Google Scholar 

  • Trdan, S., M. Vidrih, N. Valič, Ž. Laznik, 2008: Impact of entomopathogenic nematodes on adults of Phyllotreta spp. (Coleoptera: Chrysomelidae) under laboratory conditions. Acta Agric. Scand. B Soil Plant. Sci. 58, 169–175.

    Google Scholar 

  • Trdan, S., M. Vidrih, L. Andjus, Ž. Laznik, 2009: Activity of four entomopathogenic nematode species against different developmental stages of Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera, Chrysomelidae). Helminthologia 46, 14–20.

    Article  Google Scholar 

  • Unruh, T.R., L.A. Lacey, 2001: Control of codling moth, Cydia pomonella (Lepidoptera: Tortricidae), with Steinernema carpocapsae: effects of supplemental wetting and pupation site on infection rate. Biol. Control 20, 48–56.

    Article  Google Scholar 

  • Welch, H.E., L.J. Briand, 1961: Tests of the nematode DD136 and an associated bacterium for control of the Colorado potato beetle, Leptinotarsa decemlineata (Say). Can. Entomol. 93, 759–763.

    Article  Google Scholar 

  • Whalon, M.E., D.L. Miller, R.M. Hollingworth, E.J. Grafius, J.R. Miller, 1993: Selection of a Colorado potato beetle (Coleoptera: Chrysomelidae) strain resistant to Bacillus thuringiensis. J. Econ. Entomol. 86, 226–233.

    Article  Google Scholar 

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Laznik, Ž., Tóth, T., Lakatos, T. et al. Control of the Colorado potato beetle (Leptinotarsa decemlineata [Say]) on potato under field conditions: a comparison of the efficacy of foliar application of two strains of Steinernema feltiae (Filipjev) and spraying with thiametoxam. J Plant Dis Prot 117, 129–135 (2010).

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